ArticleThe Journal of cell biology1993
Correctly sorted molecules of a GPI-anchored protein are clustered and immobile when they arrive at the apical surface of MDCK cells.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 114 citations in OpenAlex.
- Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-Color Fluorescence Lifetime Cross-Correlation Spectroscopy In Vivo.Biophysical journal · 2020Article
- Glycosylphosphatidylinositol (GPI) Modification Serves as a Primary Plasmodesmal Sorting Signal.Plant physiology · 2016Article
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Golgi sorting regulates organization and activity of GPI proteins at apical membranes.Nature chemical biology · 2014Article
- Organization and execution of the epithelial polarity programme.Nature reviews. Molecular cell biology · 2014Review
- Polarized sorting and trafficking in epithelial cells.Cell research · 2012Review
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Identification of a cytoplasmic signal for apical transcytosis.Traffic (Copenhagen, Denmark) · 2009Article
- A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.The Journal of cell biology · 2009Article
- Review
- Coordinated protein sorting, targeting and distribution in polarized cells.Nature reviews. Molecular cell biology · 2008Review
- Receptor complexes cotransported via polarized endocytic pathways form clusters with distinct organizations.Molecular biology of the cell · 2007Article
- A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.Molecular biology of the cell · 2007Article
- Vectorial insertion of apical and basolateral membrane proteins in polarized epithelial cells revealed by quantitative 3D live cell imaging.The Journal of cell biology · 2006Article
- Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins.The Journal of cell biology · 2004Article
- Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.The Biochemical journal · 2001Article
- Mutant RBL mast cells defective in Fc epsilon RI signaling and lipid raft biosynthesis are reconstituted by activated Rho-family GTPases.Molecular biology of the cell · 2000Article
- Plasma membrane microdomains act as concentration platforms to facilitate intoxication by aerolysin.The Journal of cell biology · 1999Article
- N-Glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells.The Journal of cell biology · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glycosyl-phosphatidylinositol (GPI)-anchored proteins are sorted to the apical surface of many epithelial cell types. To better understand the mechanism for apical segregation of these proteins, we analyzed the lateral mobility and molecular associations of a model GPI-anchored protein, herpes simplex virus gD1 fused to human decay accelerating factor (gD1-DAF) (Lisanti, M. P., I. W. Caras, M. A. Davitz, and E. Rodriguez-Boulan. 1989. J. Cell Biol. 109:2145-2156) shortly after arrival and after long-term residence at the surface of confluent, polarized MDCK cells. FRAP measurements of lateral diffusion showed that the mobile fraction of newly arrived gD1-DAF molecules was much less than the mobile fraction of long-term resident molecules (40 vs. 80-90%). Fluorescence resonance energy transfer measurements showed that the newly arrived molecules were clustered, while resident molecules were not. Newly delivered gD1-DAF molecules were clustered but not immobilized in mutant, Concanavalin A-resistant MDCK cells that failed to sort gD1-DAF. Our results indicate that GPI-anchored proteins in MDCK cells are clustered before delivery to the surface. However, clustering alone does not target molecules for apical delivery. The immobilization observed when gD1-DAF is correctly sorted suggests that the clusters must associate some component of the cell's cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.