ArticleProceedings of the National Academy of Sciences of the United States of America1993
Changes in intracellular localization of proteasomes in immortalized ovarian granulosa cells during mitosis associated with a role in cell cycle control.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 123 citations in OpenAlex.
- Proteasome dynamics in response to metabolic changes.Frontiers in cell and developmental biology · 2025Review
- Centrosomal enrichment and proteasomal degradation of SYS-1/β-catenin requires the microtubule motor dynein.Molecular biology of the cell · 2022Article
- Multi-Step Ubiquitin Decoding Mechanism for Proteasomal Degradation.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Frontiers in plant science · 2019Article
- The Use of Proteomic Tools to Address Challenges Faced in Clonal Propagation of Tropical Crops through Somatic Embryogenesis.Proteomes · 2018Review
- The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.The Journal of cell biology · 2017Article
- Degradation-mediated protein quality control at the inner nuclear membrane.Nucleus (Austin, Tex.) · 2016Review
- Intracellular Dynamics of the Ubiquitin-Proteasome-System.F1000Research · 2015Review
- Nuclear transport of yeast proteasomes.Biomolecules · 2014Review
- Assembly of the 20S proteasome.Biochimica et biophysica acta · 2014Review
- JAMP optimizes ERAD to protect cells from unfolded proteins.Molecular biology of the cell · 2008Article
- Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis.Biological chemistry · 2008Review
- Optimizing the protein switch: altering nuclear import and export signals, and ligand binding domain.Journal of controlled release : official journal of the Controlled Release Society · 2007Article
- Localization of proteasomes and proteasomal proteolysis in the mammalian interphase cell nucleus by systematic application of immunocytochemistry.Histochemistry and cell biology · 2007Article
- Anthracyclines, proteasome activity and multi-drug-resistance.BMC cancer · 2005Article
- Subcellular recruitment of fibrillarin to nucleoplasmic proteasomes: implications for processing of a nucleolar autoantigen.Molecular biology of the cell · 2002Article
- Article
- Clastosome: a subtype of nuclear body enriched in 19S and 20S proteasomes, ubiquitin, and protein substrates of proteasome.Molecular biology of the cell · 2002Article
- Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.Molecular and cellular biology · 2001Article
- The 26S proteasome of the fission yeast Schizosaccharomyces pombe.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the isolation and characterization of proteasomes from recently established immortalized ovarian granulosa cell lines and their intracellular distribution during mitosis and during cAMP-induced differentiation, as revealed by immunofluorescence microscopy. In interphase, proteasomes were localized in small clusters throughout the cytoplasm and the nuclear matrix. In prophase, a substantial increase in proteasomal staining was observed in the perichromosomal area. A dramatic increase occurred in metaphase and in early anaphase; the chromosomes remained unstained. In late anaphase, intensive staining remained associated mainly with the spindle fibers. In telophase and early interphase of the daughter cells, intensive staining of proteasomes persisted in the nuclei. In contrast, in cells stimulated to differentiate by forskolin, which substantially elevates intracellular cAMP in these cell lines, only a weak staining of proteasomes was revealed in both the nucleus and the cytoplasm. Double staining of nondividing cells with antibodies to proteasomes and to tubulin did not show colocalization of proteasomes and microtubules. In contrast, dividing cells show a preferential concentration of proteasomes around spindle microtubules during metaphase and anaphase. The observed spatial and temporal distribution pattern of proteasomes during mitosis is highly reminiscent of the behavior of cyclins [Pines, J. & Hunter, T. (1991) J. Cell Biol. 115, 1-17]. Since proteasome accumulation appears to coincide with disappearance of cyclins A and B1 from the spindle apparatus, it is suggested that proteasomes may play a role in termination of mitosis by degrading the cyclins, which act as regulatory elements.
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