Evidence map›Paper›PMID 8380084›Full record

ArticleJournal of virology1993

Identification of alternatively spliced mRNAs encoding potential new regulatory proteins in cattle infected with bovine leukemia virus.

S Alexandersen, S Carpenter, J Christensen, T Storgaard, B Viuff, Y Wannemuehler, J Belousov, J A Roth

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 58 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

S AlexandersenDepartment of Pharmacology and Pathobiology, Royal Veterinary and Agricultural University of Copenhagen, Frederiksberg, Denmark.
S Carpenter
J Christensen
T Storgaard
B Viuff
Y Wannemuehler
J Belousov
J A Roth
Department of Agricultural Sciences · RU

Funding

BOVINE LENTIVIRUS AS A MODEL FOR HIV INFECTIONR01CA050159 · NCI · IOWA STATE UNIVERSITY · PI ROTH, JAMES A · 1989 to 1993
–
NCI NIH HHS CA50159
6 · The paper itself

Abstract

The polymerase chain reaction was used to detect and characterize low-abundance bovine leukemia virus (BLV) mRNAs. In infected cattle we could detect spliced mRNA with a splice pattern consistent with a Tax/Rex mRNA, as well as at least four alternatively spliced RNAs. Two of the alternatively spliced mRNAs encoded hitherto unrecognized BLV proteins, designated RIII and GIV. The Tax/Rex and alternatively spliced mRNAs could be detected at their highest levels in BLV-infected cell cultures; the next highest levels were found in samples from calves experimentally infected at 6 weeks postinoculation. Alternatively spliced mRNAs were also expressed, albeit at lower levels, in naturally infected animals; they were detected by a nested polymerase chain reaction. Interestingly, the GIV mRNA was specifically detected in naturally infected cows with persistent lymphocytosis and in two of five calves at 6 months after experimental infection with BLV. Furthermore, the calf with the strongest signal for GIV had the highest lymphocyte counts. These data may suggest a correlation between expression of the GIV product and development of persistent lymphocytosis. Some of the donor and acceptor sites in the alternatively spliced mRNAs were highly unusual. The biological mechanisms and significance of such a choice of unexpected splice sites are currently unknown.

Indexed as

Alternative SplicingAmino Acid SequenceAnimalsBase SequenceCattleCell-Free SystemCloning, MolecularEnzootic Bovine LeukosisGene Expression Regulation, ViralGenes, pXLeukemia Virus, BovineMolecular Sequence DataOpen Reading FramesPolymerase Chain ReactionPrecipitin TestsProtein BiosynthesisRNA, MessengerRNA, ViralViral Regulatory and Accessory Proteins

Identifiers

PMID8380084
PMCPMC237335
OpenAlexW2116954598

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.