ArticleThe Journal of cell biology1993
Assembly of type IV neuronal intermediate filaments in nonneuronal cells in the absence of preexisting cytoplasmic intermediate filaments.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 266 citations in OpenAlex.
- Neurofilaments in health and Charcot-Marie-Tooth disease.Frontiers in cell and developmental biology · 2023Review
- Overexpression of the dystrophins Dp40 and Dp40Scientific reports · 2022Article
- A review and analysis of the clinical literature on Charcot-Marie-Tooth disease caused by mutations in neurofilament protein L.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Neurofilament Light Regulates Axon Caliber, Synaptic Activity, and Organelle Trafficking in Cultured Human Motor Neurons.Frontiers in cell and developmental biology · 2021Article
- Functional Characterization of Neurofilament Light Splicing and Misbalance in Zebrafish.Cells · 2020Article
- Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Prion-like transmission of α-synuclein pathology in the context of an NFL null background.Neuroscience letters · 2017Article
- Live-cell imaging of neurofilament transport in cultured neurons.Methods in cell biology · 2016Article
- Biomarkers in biological fluids for dementia with Lewy bodies.Alzheimer's research & therapy · 2014Review
- Liquid crystal assemblies in biologically inspired systems.Liquid crystals · 2013Article
- Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- A neuronal death model: overexpression of neuronal intermediate filament protein peripherin in PC12 cells.Journal of biomedical science · 2012Article
- Article
- Article
- Assembly properties of lamprey neurofilament subunits and their expression after spinal cord transection.The Journal of comparative neurology · 2011Article
- Single-stranded noncoding RNAs mediate local epigenetic alterations at gene promoters in rat cell lines.The Journal of biological chemistry · 2011Article
- Muscle pathology without severe nerve pathology in a new mouse model of Charcot-Marie-Tooth disease type 2E.Human molecular genetics · 2011Article
- Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly.Experimental & molecular medicine · 2011Article
- Multiple neurofilament subunits are present in lamprey CNS.Brain research · 2011Article
- MSC p43 required for axonal development in motor neurons.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We report here on the in vivo assembly of alpha-internexin, a type IV neuronal intermediate filament protein, in transfected cultured cells, comparing its assembly properties with those of the neurofilament triplet proteins (NF-L, NF-M, and NF-H). Like the neurofilament triplet proteins, alpha-internexin coassembles with vimentin into filaments. To study the assembly characteristics of these proteins in the absence of a preexisting filament network, transient transfection experiments were performed with a non-neuronal cell line lacking cytoplasmic intermediate filaments. The results showed that only alpha-internexin was able to self-assemble into extensive filamentous networks. In contrast, the neurofilament triplet proteins were incapable of homopolymeric assembly into filamentous arrays in vivo. NF-L coassembled with either NF-M or NF-H into filamentous structures in the transfected cells, but NF-M could not form filaments with NF-H. alpha-internexin could coassemble with each of the neurofilament triplet proteins in the transfected cells to form filaments. When all but 2 and 10 amino acid residues were removed from the tail domains of NF-L and NF-M, respectively, the resulting NF-L and NF-M deletion mutants retained the ability to coassemble with alpha-internexin into filamentous networks. These mutants were also capable of forming filaments with other wild-type neurofilament triplet protein subunits. These results suggest that the tail domains of NF-L and NF-M are dispensable for normal coassembly of each of these proteins with other type IV intermediate filament proteins to form filaments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.