ArticleThe Journal of cell biology1993
Synchrony of cell spreading and contraction force as phagocytes engulf large pathogens.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT).Biosensors & bioelectronics · 2024Article
- Perspective: The viscoelastic properties of biofilm infections and mechanical interactions with phagocytic immune cells.Frontiers in cellular and infection microbiology · 2023Review
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Distinct timing of neutrophil spreading and stiffening during phagocytosis.Biophysical journal · 2022Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Assaying How Phagocytic Success Depends on the Elasticity of a Large Target Structure.Biophysical journal · 2019Article
- Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis.Nature communications · 2019Article
- Neutrophil Cell Shape Change: Mechanism and Signalling during Cell Spreading and Phagocytosis.International journal of molecular sciences · 2019Review
- Elastomeric sensor surfaces for high-throughput single-cell force cytometry.Nature biomedical engineering · 2018Article
- FLECS Technology for High-Throughput Single-Cell Force Biology and Screening.Assay and drug development technologies · 2018Review
- Human Astrocytes Transfer Aggregated Alpha-Synuclein via Tunneling Nanotubes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Micropipette force probe to quantify single-cell force generation: application to T-cell activation.Molecular biology of the cell · 2017Article
- How Tissue Mechanical Properties Affect Enteric Neural Crest Cell Migration.Scientific reports · 2016Article
- Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A method for time-resolved measurements of the mechanics of phagocytic cups.Journal of the Royal Society, Interface · 2013Article
- Blurred line between chemotactic chase and phagocytic consumption: an immunophysical single-cell perspective.Journal of cell science · 2011Review
- Force generation upon T cell receptor engagement.PloS one · 2011Article
- Actin switches in phagocytosis.Communicative & integrative biology · 2011Article
- Curvature recognition and force generation in phagocytosis.BMC biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple micromechanical method has been used to directly measure the force of contraction in single mammalian phagocytes (blood granulocytes) during engulfment of large yeast pathogens. Both the time course of cell spreading over the yeast particle and increase in cell body contractile force were quantitated at three temperatures in the range of 23-35 degrees C. The surprising feature of the phagocyte response was that engulfment and cell body contraction occurred in a serial sequence: i.e., the phagocyte spread rapidly over the particle at a steady rate with no detectable cell body contraction; when spreading stopped, contraction force in the cell body then rose steadily to a plateau level that remained stationary until the next sequence of spreading and contraction. Both spreading and contraction exhibited abrupt start/stop kinetics. Also impressive, the cell contraction force stimulated by phagocytosis was quite large (approximately 10(-8) N)-two orders of magnitude larger than the force necessary to deform passive phagocytes to the same extent. If distributed uniformly over the cell cross section, the contraction force is equivalent to an average contractile stress of approximately 10(3) N/m2 (0.01 Atm). These physical measurements in situ set critical requirements for the mechanism of force generation in granulocytes, imply that a major increase in network cross-linking accompanies build-up in contractile force and that subsequent network dissolution is necessary for locomotion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.