ArticleChromosoma1993
Chromosome length and DNA loop size during early embryonic development of Xenopus laevis.
Article in Chromosoma, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Regulation of the mitotic chromosome folding machines.The Biochemical journal · 2022Article
- Article
- Chromosome Compaction by Active Loop Extrusion.Biophysical journal · 2016Article
- Biological Scaling Problems and Solutions in Amphibians.Cold Spring Harbor perspectives in biology · 2015Review
- Intranuclear DNA density affects chromosome condensation in metazoans.Molecular biology of the cell · 2013Article
- Mitotic chromosome size scaling in Xenopus.Cell cycle (Georgetown, Tex.) · 2011Article
- The relative ratio of condensin I to II determines chromosome shapes.Genes & development · 2011Article
- Article
- Temporal differences in DNA replication during the S phase using single fiber analysis of normal human fibroblasts and glioblastoma T98G cells.Cell cycle (Georgetown, Tex.) · 2009Article
- Micromechanical studies of mitotic chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Review
- The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.Chromosoma · 2007Article
- Review
- Histone H1 reduces the frequency of initiation in Xenopus egg extract by limiting the assembly of prereplication complexes on sperm chromatin.Molecular biology of the cell · 1998Article
- Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.The Journal of cell biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The looped organization of the eukaryotic genome mediated by a skeletal framework of non-histone proteins is conserved throughout the cell cycle. The radial loop/scaffold model envisages that the higher order architecture of metaphase chromosomes relies on an axial structure around which looped DNA domains are radially arranged through stable attachment sites. In this light we investigated the relationship between the looped organization and overall morphology of chromosomes. In developing Xenopus laevis embryos at gastrulation, the bulk of the loops associated with histone-depleted nuclei exhibit a significant size increase, as visualized by fluorescence microscopy of the fully extended DNA halo surrounding high salt treated, ethidium bromide stained nuclei. This implies a reduction in the number of looped domains anchored to the supporting nucleoskeletal structure. The cytological analysis of metaphase plates from acetic acid fixed whole embryos, carried out in the absence of drugs inducing chromosome condensation, reveals a progressive thickening and shortening of metaphase chromosomes during development. We interpret these findings as a strong indication that the size and number of DNA loops influence the thickness and length of the chromosomes, respectively. The quantitative analysis of chromosome length distributions at different developmental stages suggests that the shortening is timed differently in different embryonic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.