ArticleJournal of virology1993
Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells.
Article in Journal of virology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants.PLoS pathogens · 2026Article
- Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants.bioRxiv : the preprint server for biology · 2026Article
- Replication-competent adenovirus reporters utilizing endogenous viral expression architecture.Journal of virology · 2025Article
- Article
- Morphological, Biochemical, and Functional Study of Viral Replication Compartments Isolated from Adenovirus-Infected Cells.Journal of virology · 2016Article
- Aggregation properties of the small nuclear ribonucleoprotein U1-70K in Alzheimer disease.The Journal of biological chemistry · 2014Article
- Viral and Cellular Components of AAV2 Replication Compartments.The open virology journal · 2013Article
- RUNX1 permits E4orf6-directed nuclear localization of the adenovirus E1B-55K protein and associates with centers of viral DNA and RNA synthesis.Journal of virology · 2008Article
- Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway.Journal of virology · 2000Article
- Adenovirus preterminal protein binds to the CAD enzyme at active sites of viral DNA replication on the nuclear matrix.Journal of virology · 1998Article
- Localization of human cytomegalovirus structural proteins to the nuclear matrix of infected human fibroblasts.Journal of virology · 1998Article
- The tripartite leader sequence of subgroup C adenovirus major late mRNAs can increase the efficiency of mRNA export.Journal of virology · 1998Article
- Targeting of U2AF65 to sites of active splicing in the nucleus.The Journal of cell biology · 1997Article
- Nuclear sites of herpes simplex virus type 1 DNA replication and transcription colocalize at early times postinfection and are largely distinct from RNA processing factors.Journal of virology · 1997Article
- Redistribution of the delta antigens in cells replicating the genome of hepatitis delta virus.Journal of virology · 1996Article
- Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain.The Journal of cell biology · 1996Article
- The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition.The Journal of cell biology · 1996Article
- Herpes simplex virus type 1 protein IE63 affects the nuclear export of virus intron-containing transcripts.Journal of virology · 1996Article
- The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.Molecular biology of the cell · 1996Article
- Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei.The Journal of cell biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the effect of adenovirus infection on the nuclear organization of splicing small nuclear ribonucleoproteins (snRNPs) in HeLa cells. In uninfected HeLa cells, snRNPs are widespread throughout the nucleoplasm but also are concentrated in specific nuclear structures, including coiled bodies, interchromatin granules, and perichromatin fibrils. We have used immunofluorescence microscopy to study the localization of splicing snRNPs relative to centers of viral DNA synthesis and accumulation identified with antiserum against the viral 72,000-molecular-weight single-stranded DNA-binding protein (72K protein). Splicing snRNPs were independently detected with both monoclonal and polyclonal antibodies specific for common snRNP antigens, snRNP-specific proteins, and the snRNA-specific 2,2,7-trimethylguanosine 5' cap structure. We have examined infected cells 2 to 24 h after infection, and, in the majority of these cells, we observed no colocalization of the snRNP and 72K-protein staining patterns. In the late phase, snRNPs were found to markedly concentrate in discrete clusters that were distinct from the centers of viral DNA synthesis and accumulation identified with anti-72K protein. We have treated cells with hydroxyurea at various times after infection to inhibit aspects of the virus infectious program. We have found that the accumulation of snRNP clusters is correlated with late gene expression rather than with DNA synthesis or early gene expression. Finally, we show that the late-phase snRNP clusters colocalize with a monoclonal antibody that primarily stains interchromatin granules. These results suggest that the centers of snRNP concentration in late-phase infected cells are likely to correspond to interchromatin granule clusters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.