ArticleProceedings of the National Academy of Sciences of the United States of America1993
Control analysis of the dependence of Escherichia coli physiology on the H(+)-ATPase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Escherichia coli robustly expresses ATP synthase at growth rate-maximizing concentrations.The FEBS journal · 2022Article
- Salmonella Central Carbon Metabolism Enhances Bactericidal Killing by Fluoroquinolone Antibiotics.Antimicrobial agents and chemotherapy · 2022Article
- Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies.Nature communications · 2022Article
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- Searching for principles of microbial physiology.FEMS microbiology reviews · 2020Review
- Enhanced Glucose Consumption and Organic Acid Production by Engineered Corynebacterium glutamicum Based on Analysis of a pfkB1 Deletion Mutant.Applied and environmental microbiology · 2017Article
- Optimality and sub-optimality in a bacterial growth law.Nature communications · 2017Article
- Optimality principles in the regulation of metabolic networks.Metabolites · 2012Article
- Systems biology of lactic acid bacteria: a critical review.Microbial cell factories · 2011Review
- Glyceraldehyde-3-phosphate dehydrogenase has no control over glycolytic flux in Lactococcus lactis MG1363.Journal of bacteriology · 2003Article
- The glycolytic flux in Escherichia coli is controlled by the demand for ATP.Journal of bacteriology · 2002Article
- The extent to which ATP demand controls the glycolytic flux depends strongly on the organism and conditions for growth.Molecular biology reports · 2002Article
- Subtleties in control by metabolic channelling and enzyme organization.Molecular and cellular biochemistry · 1998Article
- Metabolic engineering of sugar catabolism in lactic acid bacteria.Antonie van Leeuwenhoek · 1996Review
- Experimental determination of control by the H(+)-ATPase in Escherichia coli.Journal of bioenergetics and biomembranes · 1995Review
- Elusive control.Journal of bioenergetics and biomembranes · 1995Review
- Energy buffering of DNA structure fails when Escherichia coli runs out of substrate.Journal of bacteriology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The H(+)-ATPase plays a central role in Escherichia coli free-energy transduction and hence in E. coli physiology. We here investigate the extent to which this enzyme also controls the growth rate, growth yield, and respiratory rate of E. coli. We modulate the expression of the atp operon and determine the effect on said properties. When quantified in terms of control coefficients, we find that, in the wild-type cell growing on glucose in minimal medium, this key enzyme (H(+)-ATPase) exerts virtually no control on growth rate (magnitude of C < 0.01), a minor positive control on growth yield (C = 0.15), and a small but negative control on respiration rate (C = -0.25). The control the enzyme exerts on the consumption rate of the carbon and free-energy substrate is negative (C = -0.15). We also studied how the control coefficients themselves vary with the expression of the atp operon. As the level of expression of the atp operon was reduced, the control exerted by the H(+)-ATPase on growth rate and growth yield increased slightly; the control on growth rate passed through a maximum (C = 0.1) and disappeared when the atp operon was not expressed at all, reflecting that with this substrate there are alternative routes for ATP synthesis. At elevated levels of the H(+)-ATPase compared to the wild type, the control exerted by the enzyme on growth rate became negative. The evolutionary context of the absence of control by the atp operon on growth rate is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.