ArticleNucleic acids research1993
Fission yeast with DNA polymerase delta temperature-sensitive alleles exhibits cell division cycle phenotype.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.Nucleic acids research · 2026Article
- The kinase domain residue serine 173 ofBiology open · 2017Article
- A panoply of errors: polymerase proofreading domain mutations in cancer.Nature reviews. Cancer · 2016Review
- Meiotic recombination protein Rec12: functional conservation, crossover homeostasis and early crossover/non-crossover decision.Nucleic acids research · 2011Article
- Article
- Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis.BMC molecular biology · 2009Article
- Article
- Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.Nucleic acids research · 2005Article
- Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27.BMC molecular biology · 2004Article
- The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit.Nucleic acids research · 2004Article
- Replication proteins influence the maintenance of telomere length and telomerase protein stability.Molecular and cellular biology · 2003Article
- Distinct functions of S. pombe Rec12 (Spo11) protein and Rec12-dependent crossover recombination (chiasmata) in meiosis I; and a requirement for Rec12 in meiosis II.Cell & chromosome · 2002Article
- Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that dna2 plays an essential role in Okazaki fragment metabolism.Genetics · 2000Article
- Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.Molecular and cellular biology · 2000Article
- Novel WD-repeat protein Mip1p facilitates function of the meiotic regulator Mei2p in fission yeast.Molecular and cellular biology · 2000Article
- Fission yeast Pob1p, which is homologous to budding yeast Boi proteins and exhibits subcellular localization close to actin patches, is essential for cell elongation and separation.Molecular biology of the cell · 1999Article
- Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.Genetics · 1999Article
- Mutator phenotype induced by aberrant replication.Molecular and cellular biology · 1999Article
- Mutant DNA polymerase delta from thermosensitive Schizosaccharomyces pombe strains display reduced stimulation by proliferating cell nuclear antigen.The Biochemical journal · 1998Article
- Mutational effect of fission yeast polalpha on cell cycle events.Molecular biology of the cell · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
DNA polymerases alpha and delta are essential enzymes believed to play critical roles in initiation and replication of chromosome DNA. In this study, we show that the genes for Schizosaccharomyces pombe (S.pombe) DNA polymerase alpha and delta (pol alpha+ and pol delta+) are essential for cell viability. Disruption of either the pol alpha+ or pol delta+ gene results in distinct terminal phenotypes. The S.pombe pol delta+ gene is able to complement the thermosensitive cdc2-2 allele of Saccharomyces cerevisiae (S.cerevisiae) at the restrictive temperature. By random mutagenesis in vitro, we generated three pol delta conditional lethal alleles. We replaced the wild type chromosomal copy of pol delta+ gene with the mutagenized sequence and characterized the thermosensitive alleles in vivo. All three thermosensitive mutants exhibit a typical cell division cycle (cdc) terminal phenotype similar to that of the disrupted pol delta+ gene. Flow cytometric analysis showed that at the nonpermissive temperature all three mutants were arrested in S phase of the cell cycle. The three S.pombe conditional pol delta alleles were recovered and sequenced. The mutations causing the thermosensitive phenotype are missense mutations. The altered amino acid residues are uniquely conserved among the known polymerase delta sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.