ArticleInfection and immunity1993
Structural analysis of the Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI) operon.
Article in Infection and immunity, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Construction and immunization with double mutant ΔJournal of veterinary science · 2020Article
- Proteomic Analysis of Novel Components ofToxins · 2019Article
- Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil.PloS one · 2018Article
- Identification and characterization of a repeat-in-toxin gene cluster in Vibrio anguillarum.Infection and immunity · 2008Article
- ohr, Encoding an organic hydroperoxide reductase, is an in vivo-induced gene in Actinobacillus pleuropneumoniae.Infection and immunity · 2002Article
- Detection of RTX toxin genes in gram-negative bacteria with a set of specific probes.Applied and environmental microbiology · 1997Article
- Actinobacillus suis strains isolated from healthy and diseased swine are clonal and carry apxICABDvar. suis and apxIICAvar. suis toxin genes.Journal of clinical microbiology · 1997Article
- Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933.Infection and immunity · 1995Article
- Knockout mutants of Actinobacillus pleuropneumoniae serotype 1 that are devoid of RTX toxins do not activate or kill porcine neutrophils.Infection and immunity · 1995Article
- RTX toxin genotypes and phenotypes in Actinobacillus pleuropneumoniae field strains.Journal of clinical microbiology · 1994Article
- Genetic map of the Actinobacillus pleuropneumoniae RTX-toxin (Apx) operons: characterization of the ApxIII operons.Infection and immunity · 1994Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Actinobacillus pleuropneumoniae-RTX-toxin I (ApxI), an important virulence factor, is secreted by serotypes 1, 5, 9, 10, and 11 of A. pleuropneumoniae. However, sequences homologous to the secretion genes apxIBD of the ApxI operon are present in all 12 serotypes except serotype 3. The purpose of this study was to determine and compare the structures of the ApxI operons of the 12 A. pleuropneumoniae serotypes. We focused on the nucleotide sequence comparison of the ApxI-coding genes, the structures of the ApxI operons, and the transcription of the ApxI operons. We determined the nucleotide sequences of the toxin-encoding apxICA genes of serotype 9 and found that the gene for the structural toxin, apxIA, was almost identical to the apxIA gene of serotype 1. The toxin-encoding genes of the other serotypes are also similar for the main part; nevertheless, two variants were identified, one in serotypes 1, 9, and 11 and one in serotypes 5 and 10. The two apxIA variants differ mainly within the distal 110 nucleotides. Structural analysis demonstrated that intact ApxI operons, consisting of the four contiguous genes apxICABD, are present in serotypes 1, 5, 9, 10, and 11. ApxI operons with a major deletion in the apxICA genes are present in serotypes 2, 4, 6, 7, 8, and 12. Serotype 3 does not contain ApxI operon sequences. We found that all ApxI operons are transcriptionally active despite the partial deletion of the operon in some serotypes. The implications of these data for the expression and secretion of ApxI and the other Apx-toxins, ApxII and ApxIII, as well as for the development of a subunit vaccine against A. pleuropneumoniae will be discussed.
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