ArticlePlant molecular biology1993
The U6 small nuclear RNA gene family of potato.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Article
- High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato.Scientific reports · 2019Article
- The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato.Plant cell reports · 2019Article
- Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts.Plant cell reports · 2017Article
- Expression of tandem invertase genes associated with sexual and vegetative growth cycles in potato.Plant molecular biology · 1999Article
- Molecular analysis of the gene family of the signal recognition particle (SRP) RNA of tomato.Plant molecular biology · 1996Article
- Small RNA database.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the inverse polymerase chain reaction (IPCR), 19 U6snRNA gene promoters were isolated from the potato genome. Analysis of their nucleotide sequences revealed the existence of two subfamilies. Promoters from class 1 harbour the typical sequence elements required for plant snRNA gene transcription whereas those from class 2 do not have a TATA box. Three promoters were fused to a modified U6snRNA-coding sequence to allow their activity to be monitored in tobacco protoplasts. Two of the promoters, one from either class, were found to be active. Comparison of potato U6snRNA gene promoter sequences with those found in other plant species showed various degrees of homology. In addition, the entire nucleotide sequences of seven potato U6snRNA genes and one pseudogene were determined. The overall frequency of nucleotide changes after PCR was found to be 1.15 x 10(-3). The mutations appeared to be clustered in a distinct area and were all A-to-G/T-to-C substitutions.
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Registered trials
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