ArticleProceedings of the National Academy of Sciences of the United States of America1993
Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
73 citing papers in PubMed, 264 citations in OpenAlex.
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- Chimeric antigen receptor T cells march into T cell malignancies.Journal of cancer research and clinical oncology · 2023Review
- Intracellular Antibodies for Drug Discovery and as Drugs of the Future.Antibodies (Basel, Switzerland) · 2023Review
- A platform for post-translational spatiotemporal control of cellular proteins.Synthetic biology (Oxford, England) · 2021Article
- A novel method to generate T-cell receptor-deficient chimeric antigen receptor T cells.Blood advances · 2018Article
- Implication of Soluble Forms of Cell Adhesion Molecules in Infectious Disease and Tumor: Insights from Transgenic Animal Models.International journal of molecular sciences · 2018Review
- Intrabody targeting vascular endothelial growth factor receptor-2 mediates downregulation of surface localization.Cancer gene therapy · 2017Article
- Intracellular targeting with engineered proteins.F1000Research · 2016Review
- Epigenetic Regulation of Antibody Responses by the Histone H2A Deubiquitinase MYSM1.Scientific reports · 2015Article
- Reduction of HIV-1 infectivity through endoplasmic reticulum-associated degradation-mediated Env depletion.Journal of virology · 2015Article
- Article
- Targeted in vivo inhibition of specific protein-protein interactions using recombinant antibodies.PloS one · 2014Article
- Single domain intrabodies against WASP inhibit TCR-induced immune responses in transgenic mice T cells.Scientific reports · 2013Article
- Immunosympathectomy as the first phenotypic knockout with antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The use of phage display to generate conformation-sensor recombinant antibodies.Nature protocols · 2012Article
- Zinc finger protein designed to target 2-long terminal repeat junctions interferes with human immunodeficiency virus integration.Human gene therapy · 2012Article
- Anti-CHMP5 single chain variable fragment antibody retrovirus infection induces programmed cell death of AML leukemic cells in vitro.Acta pharmacologica Sinica · 2012Article
- Baculovirus display of single chain antibody (scFv) using a novel signal peptide.BMC biotechnology · 2010Article
- Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.Protein engineering, design & selection : PEDS · 2010Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
A single-chain antibody, derived from a human monoclonal antibody that recognizes the CD4 binding region of the human immunodeficiency virus type 1 (HIV-1) envelope protein, has been designed for intracellular expression in eukaryotic cells. The single-chain antibody is composed of an immunoglobulin heavy-chain leader sequence and heavy- and light-chain variable regions that are joined by an interchain linker. The antibody is stably expressed and retained in the endoplasmic reticulum and is not toxic to the cells. The antibody binds to the envelope protein within the cell and inhibits processing of the envelope precursor and syncytia formation. The infectivity of the HIV-1 particles produced by cells that express the single-chain antibody is substantially reduced. These studies illustrate the feasibility of designing antibodies that bind and inactivate molecules intracellularly. Antibodies that act on target molecules within cells should provide a useful tool for research as well as for control of infectious and other diseases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.