ArticleMolecular and cellular biology1993
Transcriptional regulator Leu3 of Saccharomyces cerevisiae: separation of activator and repressor functions.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production.Nature communications · 2022Article
- InFrontiers in microbiology · 2020Article
- Multidrug resistance in fungi: regulation of transporter-encoding gene expression.Frontiers in physiology · 2014Review
- Development of a chromosomally integrated metabolite-inducible Leu3p-alpha-IPM "off-on" gene switch.PloS one · 2010Article
- Dynamics and design principles of a basic regulatory architecture controlling metabolic pathways.PLoS biology · 2008Article
- Characterization of pco-1, a newly identified gene which regulates purine catabolism in Neurospora.Current genetics · 2004Article
- Leucine biosynthesis in fungi: entering metabolism through the back door.Microbiology and molecular biology reviews : MMBR · 2003Review
- Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1.Molecular and cellular biology · 1998Article
- Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators.Nucleic acids research · 1996Article
- Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p.Molecular and cellular biology · 1996Article
- Detection of leucine-independent DNA site occupancy of the yeast Leu3p transcriptional activator in vivo.Molecular and cellular biology · 1995Article
- The Saccharomyces cerevisiae Leu3 protein activates expression of GDH1, a key gene in nitrogen assimilation.Molecular and cellular biology · 1995Article
- Molecular architecture of a Leu3p-DNA complex in solution: a biochemical approach.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Leu3 protein of Saccharomyces cerevisiae binds to specific DNA sequences present in the 5' noncoding region of at least five RNA polymerase II-transcribed genes. Leu3 functions as a transcriptional activator only when the metabolic intermediate alpha-isopropylmalate is also present. In the absence of alpha-isopropylmalate, Leu3 causes transcription to be repressed below basal levels. We show here that different portions of the Leu3 protein are responsible for activation and repression. Fusion of the 30 C-terminal residues of Leu3 to the DNA-binding domain of the Gal4 protein created a strong cross-species activator, demonstrating that the short C-terminal region is not only required but also sufficient for transcriptional activation. Using a recently developed Leu3-responsive in vitro transcription assay as a test system for repression (J. Sze, M. Woontner, J. Jaehning, and G. B. Kohlhaw, Science 258:1143-1145, 1992), we show that mutant forms of the Leu3 protein that lack the activation domain still function as repressors. The shortest repressor thus identified had only about 15% of the mass of the full-length Leu3 protein and was centered on the DNA-binding region of Leu3. Implications of this finding for the mechanism of repression are discussed.
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