ArticleMolecular and cellular biology1993
The Ets-related transcription factor PU.1 immortalizes erythroblasts.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
- Arid2 regulates hematopoietic stem cell differentiation in normal hematopoiesis.Experimental hematology · 2021Article
- Erythropoietin Regulates Transcription and YY1 Dynamics in a Pre-established Chromatin Architecture.iScience · 2020Article
- The Ets2 Repressor Factor (Erf) Is Required for Effective Primitive and Definitive Hematopoiesis.Molecular and cellular biology · 2017Article
- Mechanisms of in vivo binding site selection of the hematopoietic master transcription factor PU.1.Nucleic acids research · 2013Article
- Concise review: production of cultured red blood cells from stem cells.Stem cells translational medicine · 2012Review
- Dose-dependent repression of T-cell and natural killer cell genes by PU.1 enforces myeloid and B-cell identity.Leukemia · 2008Article
- Review
- Reprogramming leukemia cells to terminal differentiation and growth arrest by RNA interference of PU.1.Molecular cancer research : MCR · 2007Article
- Expansion of CD34+ cells on telomerized human stromal cells without losing erythroid-differentiation potential in a serum-free condition.International journal of hematology · 2005Article
- Inhibition of CBP-mediated protein acetylation by the Ets family oncoprotein PU.1.Molecular and cellular biology · 2002Article
- Review
- Review
- Article
- In vivo complex formation of PU.1 with HDAC1 associated with PU.1-mediated transcriptional repression.Oncogene · 2001Article
- Loss of p53 tumor suppressor function is required for in vivo progression of Friend erythroleukemia.Oncogene · 2001Article
- Review
- Ets and retroviruses - transduction and activation of members of the Ets oncogene family in viral oncogenesis.Oncogene · 2000Review
- BSAP can repress enhancer activity by targeting PU.1 function.Molecular and cellular biology · 2000Article
- GOOSECOID inhibits erythrocyte differentiation by competing with Rb for PU.1 binding in murine cells.Oncogene · 1999Article
- Direct interaction of hematopoietic transcription factors PU.1 and GATA-1: functional antagonism in erythroid cells.Genes & development · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In vivo studies of Friend virus erythroleukemia have implied that proviral integrations adjacent to the gene for the Ets-related transcription factor PU.1 may inhibit the commitment of erythroblasts to differentiate and cause their capability for indefinite transplantation (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988; R. Paul, S. Schuetze, S. L. Kozak, C. Kozak, and D. Kabat, J. Virol. 65:464-467, 1991). To test this hypothesis, we ligated PU.1 cDNA into a retroviral vector and studied its effects on cultured cells. Infection of fibroblasts with PU.1-encoding retrovirus resulted in PU.1 synthesis followed by nuclear pyknosis, cell rounding, and degeneration. In contrast, in long-term bone marrow cultures, erythroblasts were efficiently and rapidly immortalized. The resulting cell lines were polyclonal populations that contained PU.1, were morphologically blast-like, required erythropoietin and bone marrow stromal cells for survival and proliferation, and spontaneously differentiated at low frequency to synthesize hemoglobin. After 9 months in culture, erythroblasts became stroma independent, and they then grew as clonal cell lines. We conclude that PU.1 perturbs the pathway(s) that controls potential for indefinite proliferation and that it can be used to generate permanent erythroblast cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.