ArticleMolecular and cellular biology1993
Saccharomyces cerevisiae HSP70 heat shock elements are functionally distinct.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Hsp70 chaperones, Ssa1 and Ssa2, limit poly(A) binding protein aggregation.Molecular biology of the cell · 2025Article
- Screening and Structural Characterization of Heat Shock Response Elements (HSEs) inInternational journal of molecular sciences · 2024Article
- Essentiality of Sis1, a J-domain protein Hsp70 cochaperone, can be overcome by Tti1, a specialized PIKK chaperone.Molecular biology of the cell · 2022Article
- Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco.AMB Express · 2020Article
- Identification of a Heat-Inducible Element of Cysteine Desulfurase Gene Promoter inMolecules (Basel, Switzerland) · 2019Article
- Cell-to-cell variability in the propensity to transcribe explains correlated fluctuations in gene expression.Cell systems · 2015Article
- Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae.Proteomics · 2015Article
- Genome-wide analysis reveals new roles for the activation domains of the Saccharomyces cerevisiae heat shock transcription factor (Hsf1) during the transient heat shock response.The Journal of biological chemistry · 2006Article
- Displacement of histones at promoters of Saccharomyces cerevisiae heat shock genes is differentially associated with histone H3 acetylation.Molecular and cellular biology · 2006Article
- Phosphorylation of the yeast heat shock transcription factor is implicated in gene-specific activation dependent on the architecture of the heat shock element.Molecular and cellular biology · 2004Article
- A trans-activation domain in yeast heat shock transcription factor is essential for cell cycle progression during stress.Molecular and cellular biology · 1999Article
- Heat shock element architecture is an important determinant in the temperature and transactivation domain requirements for heat shock transcription factor.Molecular and cellular biology · 1998Article
- A bipartite operator interacts with a heat shock element to mediate early meiotic induction of Saccharomyces cerevisiae HSP82.Molecular and cellular biology · 1995Article
- Heat shock and light activation of a Chlamydomonas HSP70 gene are mediated by independent regulatory pathways.Molecular & general genetics : MGG · 1995Article
- A heat shock transcription factor with reduced activity suppresses a yeast HSP70 mutant.Molecular and cellular biology · 1995Article
- Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae HSP70 gene SSA1 has multiple heat shock elements (HSEs). To determine the significance of each of these sequences for expression of SSA1, we analyzed expression from a set of promoters containing point mutations in each of the HSEs, individually and in pairwise combinations. Of the three HSE-like sequences, two (HSE2 and HSE3) were active promoter elements; only one, HSE2, was active under basal growth conditions. Either HSE2 or HSE3 alone was able to drive SSA1 transcription at near-normal rates after heat shock. Both HSE2 and HSE3 were capable of driving basal transcription when placed in the context of the CYC1 promoter. Previous analysis had identified an upstream repressing sequence overlapping HSE2 that repressed basal transcription driven by HSE2. Our analysis showed that basal transcription driven by HSE3 was repressed both by the distant upstream repressing sequence and by closer flanking sequences. The ability to drive basal transcription is not inherent in all natural HSEs, since the HSEs from the heat-inducible SSA3 and SSA4 genes showed no basal activity when placed in the CYC1 vector. Gel mobility shift experiments showed that the same population of heat shock transcription factor molecules bound to HSEs capable of driving basal activity and to HSEs having very low or undetectable basal activity.
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