ArticleMolecular and cellular biology1993
Demethylation of somatic and testis-specific histone H2A and H2B genes in F9 embryonal carcinoma cells.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- TH2A is phosphorylated at meiotic centromere by Haspin.Chromosoma · 2017Article
- Identification of a variant-specific phosphorylation of TH2A during spermiogenesis.Scientific reports · 2017Article
- Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells.Stem cells international · 2017Review
- The Effects of Chemotherapeutic Agents, Bleomycin, Etoposide, and Cisplatin, on Chromatin Remodeling in Male Rat Germ Cells.Biology of reproduction · 2016Article
- Article
- Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.Genes & development · 2013Article
- Article
- Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Trinucleotide repeats associated with human disease.Nucleic acids research · 1997Review
- Genomic analysis of the mouse protamine 1, protamine 2, and transition protein 2 gene cluster reveals hypermethylation in expressing cells.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- The CpG-specific methylase SssI has topoisomerase activity in the presence of Mg2+.Nucleic acids research · 1994Article
- A B2-L1 composite between rat histone H2A and H3 genes.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In contrast to many other genes containing a CpG island, the testis-specific H2B (TH2B) histone gene exhibits tissue-specific methylation patterns in correlation with gene activity. Characterization of the methylation patterns within a 20-kb segment containing the TH2A and TH2B genes in comparison with that in a somatic histone cluster revealed that: (i) the germ cell-specific unmethylated domain of the TH2A and TH2B genes is defined as a small region surrounding the CpG islands of the TH2A and TH2B genes and (ii) somatic histone genes are unmethylated in both liver and germ cells, like other genes containing CpG islands, whereas flanking sequences are methylated. Transfection of in vitro-methylated TH2B, somatic H2B, and mouse metallothionein I constructs into F9 embryonal carcinoma cells revealed that the CpG islands of the TH2A and TH2B genes were demethylated like those of the somatic H2A and H2B genes and the metallothionein I gene. The demethylation of those CpG islands became significantly inefficient at a high number of integrated copies and a high density of methylated CpG dinucleotides. In contrast, three sites in the somatic histone cluster, of which two sites are located in the long terminal repeat of an endogenous retrovirus-like sequence, were efficiently demethylated even at a high copy number and a high density of methylated CpG dinucleotides. These results suggest two possible mechanisms for demethylation in F9 cells and methylation of CpG islands of the TH2A and TH2B genes at the postblastula stage during embryogenesis.
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