ArticleThe Journal of clinical investigation1993
Human neutrophil-mediated fungistasis against Histoplasma capsulatum. Localization of fungistatic activity to the azurophil granules.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- How Histoplasma Evades the Human Immune System.Acta medica academica · 2025Review
- Histoplasmosis.Infectious disease clinics of North America · 2025Review
- Pathogenicity & virulence ofVirulence · 2022Review
- Neutrophil and Eosinophil DNA Extracellular Trap Formation: Lessons From Pathogenic Fungi.Frontiers in microbiology · 2021Review
- BiodiversemSphere · 2020Article
- The Role of the Interleukin-17 Axis and Neutrophils in the Pathogenesis of Endemic and Systemic Mycoses.Frontiers in cellular and infection microbiology · 2020Review
- Flying under the radar: Histoplasma capsulatum avoidance of innate immune recognition.Seminars in cell & developmental biology · 2019Review
- Histoplasma Capsulatum: Mechanisms for Pathogenesis.Current topics in microbiology and immunology · 2019Review
- Influence of Microbes on Neutrophil Life and Death.Frontiers in cellular and infection microbiology · 2017Review
- NADPH Oxidase Deficiency: A Multisystem Approach.Oxidative medicine and cellular longevity · 2017Review
- Protective Effect of Galectin-1 duringMediators of inflammation · 2016Article
- Impairment of neutrophil migration to remote inflammatory site during lung histoplasmosis.BioMed research international · 2015Article
- Celecoxib improves host defense through prostaglandin inhibition during Histoplasma capsulatum infection.Mediators of inflammation · 2013Article
- Review
- 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection.PloS one · 2012Article
- Histoplasma capsulatum manifests preferential invasion of phagocytic subpopulations in murine lungs.Journal of leukocyte biology · 2008Article
- The flagellum of Pseudomonas aeruginosa is required for resistance to clearance by surfactant protein A.PloS one · 2007Article
- Tumor necrosis factor-alpha antagonism by the murine tumor necrosis factor-alpha receptor 2-Fc fusion protein exacerbates histoplasmosis in mice.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2007Article
- Comparative signature-tagged mutagenesis identifies Pseudomonas factors conferring resistance to the pulmonary collectin SP-A.PLoS pathogens · 2005Article
- Apoptosis modulates protective immunity to the pathogenic fungus Histoplasma capsulatum.The Journal of clinical investigation · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human neutrophils (PMN) demonstrated potent fungistatic activity against Histoplasma capsulatum (Hc) yeasts in a sensitive microassay that quantifies the growth of yeasts by the incorporation of [3H]leucine. At a PMN:yeast ratio of 1:2, PMN inhibited the growth of yeasts by 37%. Maximum inhibition of 85% to 95% was achieved at a PMN/yeast ratio of 10:1 to 50:1. Opsonization of the yeasts in fresh or heat-inactivated serum was required for PMN-mediated fungistasis, but ingestion of the yeasts was not required. Recognition and phagocytosis of opsonized yeasts was via PMN complement receptor (CR) type 1 (CR1), CR3, and FcRIII (CD16). PMN fungistatic activity was evident by 2 h, was maximum at 24 h, and persisted up to 5 d. In contrast, yeasts multiplied within monocytes to a greater extent than in culture medium alone. PMN from three patients with chronic granulomatous disease (CGD) inhibited the growth of Hc yeasts by an average of 97%, compared with 86% in three normal controls. Furthermore, preincubation of PMN with the lysosomotropic agent NH4Cl inhibited fungistatic activity in a concentration-dependent manner. Finally, experiments with subcellular fractions of PMN demonstrated that the principal component of the fungistatic activity of PMN was localized in the azurophil granules. These data demonstrate that human PMN possess potent fungistatic activity against Hc yeasts and further show that fungistasis is mediated by antimicrobial agents contained in the azurophil granules.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.