ArticleGenetics1993
TSF1 to TSF6, required for silencing the Saccharomyces cerevisiae GAL genes, are global regulatory genes.
Article in Genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Transcriptome Analysis of FourG3 (Bethesda, Md.) · 2018Article
- Analysis of transcriptional activation at a distance in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Molecular genetics of the RNA polymerase II general transcriptional machinery.Microbiology and molecular biology reviews : MMBR · 1998Review
- Article
- Saccharomyces cerevisiae BUR6 encodes a DRAP1/NC2alpha homolog that has both positive and negative roles in transcription in vivo.Molecular and cellular biology · 1997Article
- SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- Paf1p, an RNA polymerase II-associated factor in Saccharomyces cerevisiae, may have both positive and negative roles in transcription.Molecular and cellular biology · 1996Article
- SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II.Molecular and cellular biology · 1996Article
- Drosophila Polycomb-group regulated chromatin inhibits the accessibility of a trans-activator to its target DNA.The EMBO journal · 1995Article
- Regulation of HIS4 expression by the Saccharomyces cerevisiae SIN4 transcriptional regulator.Genetics · 1995Article
- Article
- Differential effects of Cdc68 on cell cycle-regulated promoters in Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
- Synergistic release from glucose repression by mig1 and ssn mutations in Saccharomyces cerevisiae.Genetics · 1994Article
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6 authors at 1 institution in 1 country.
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Abstract
The Saccharomyces cerevisiae GAL1 and GAL10 genes are controlled in response to the availability of galactose and glucose by multiple activating and repressing proteins bound at adjacent or overlapping sites in UASG. Negative control elements in UASG, designated GAL operators GALO1 to GALO6, are required to silence basal level transcription of GAL1 and GAL10 when galactose is absent. We isolated and characterized recessive mutations in six nuclear genes, TSF1 to TSF6, that impair silencing of GAL1 and GAL10 gene expression. Surprisingly, the results of several experiments suggest that the TSF genes encode global regulatory factors. tsf1 to tsf6 mutations derepressed expression from yeast CYC-GAL hybrid promoters (fused to lacZ) that harbor a variety of operator sequences, and caused pleiotropic defects in cell growth, mating, and sporulation. S1 mapping and Northern blot results for tsf3 suggest that the molecular defect is at the transcriptional level. Mutant phenotypes were additive in certain combinations of tsf double mutants, implying that more than one silencing pathway is involved in TSF1 to TSF6 function. Most significantly, mutations in all six TSF1 to TSF6 genes activated expression from GAL1 and CYC1 promoters (fused to lacZ) lacking upstream activating sequences. Combined, the simplest interpretation of these results is that TSF1 to TSF6 encode factors that control the function of the basic RNA polymerase II transcriptional machinery.
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