ArticleProceedings of the National Academy of Sciences of the United States of America1993
Binding to heparan sulfate or heparin enhances neutrophil responses to interleukin 8.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers.
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Who cites it
119 citing papers in PubMed, 439 citations in OpenAlex.
- Heparan Sulfate Proteoglycans in Immunity.Proteoglycan research · 2026Article
- Blood Cells, Endothelial Cells, and Circulating Extracellular Vesicles Induce Procoagulant Activity by Phosphatidylserine Exposure in Chronic Coronary Artery Disease Patients with In-stent Restenosis after Percutaneous Coronary Intervention.Journal of atherosclerosis and thrombosis · 2026Article
- Metal-Mediated Il-8 Binding to Heparan Sulfate Evaluated by Electrochemical Impedance Spectroscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Neutrophils in Tissue Injury and Repair: Molecular Mechanisms and Therapeutic Targets.MedComm · 2025Review
- Review
- Cardiac Fibroblastic Niches in Homeostasis and Inflammation.Circulation research · 2024Review
- KRT17high/CXCL8+ Tumor Cells Display Both Classical and Basal Features and Regulate Myeloid Infiltration in the Pancreatic Cancer Microenvironment.Clinical cancer research : an official journal of the American Association for Cancer Research · 2024Article
- Unveiling cytokine charge disparity as a potential mechanism for immune regulation.Cytokine & growth factor reviews · 2024Review
- Deciphering the role of glycosaminoglycans in GPCR signaling.Cellular signalling · 2024Review
- Infant respiratory infections modulate lymphocyte homing to breast milk.Frontiers in immunology · 2024Article
- Heterogeneity in Neutrophil Extracellular Traps from Healthy Human Subjects.International journal of molecular sciences · 2023Article
- Heterogeneity in Neutrophil Extracellular Traps from Healthy Human Subjects.bioRxiv : the preprint server for biology · 2023Article
- The chemokines CXCL8 and CXCL12: molecular and functional properties, role in disease and efforts towards pharmacological intervention.Cellular & molecular immunology · 2023Review
- Heparin Specifically Interacts with Basic BBXB Motifs of the Chemokine CCL21 to Define CCR7 Signaling.International journal of molecular sciences · 2023Article
- Macrophages in myocardial infarction.American journal of physiology. Cell physiology · 2022Review
- The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis.International journal of molecular sciences · 2022Article
- Targeting of Glycosaminoglycans in Genetic and Inflammatory Airway Disease.International journal of molecular sciences · 2022Review
- Immunologic Roles of Hyaluronan in Dermal Wound Healing.Biomolecules · 2021Review
- The dimeric form of CXCL12 binds to atypical chemokine receptor 1.Science signaling · 2021Article
- Latest update on chemokine receptors as therapeutic targets.Biochemical Society transactions · 2021Review
59 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of interleukin 8 (IL-8) with heparin was studied by using synthetic IL-8 analogs with C- and N-terminal truncations. Elimination of the N-terminal region preceding the first cysteine, which constitutes the IL-8 receptor binding site, did not affect the affinity to heparin-Sepharose. Affinity, however, decreased with progressive truncation at the C terminus, and no binding was observed when the C-terminal alpha-helix was eliminated. The effect of heparin and other glycosaminoglycans on IL-8 activity was also tested. When IL-8 was applied together with heparan sulfate, neutrophil chemotaxis in vitro was enhanced up to 4-fold, and the stimulus-dependent increase in cytosolic free Ca2+ increased markedly in both rate and peak value. Heparin had a similar effect on the Ca2+ response but did not enhance chemotaxis. The glycosaminoglycans by themselves did not elicit neutrophil responses. Their enhancing effect was restricted to stimulation with IL-8 and was not observed when the unrelated chemoattractant fMet-Ile-Phe-Leu was used as the stimulus. Elastase released from stimulated neutrophils was inhibited by heparin, heparan sulfate, and, to a lesser extent, chondroitin sulfate B, confirming previous observations. Taken together, these results suggest that heparan sulfate, which is present on the endothelial cell surface and in the basement membrane, may have a dual function in diapedesis, promotion of IL-8-dependent transmigration of neutrophils, and protection of the tissue microenvironment from damage by lytic enzymes released from the migrating cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.