ArticleThe EMBO journal1993
Yeast prt1 mutations alter heat-shock gene expression through transcript fragmentation.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- P-body components, Dhh1 and Pat1, are involved in tRNA nuclear-cytoplasmic dynamics.RNA (New York, N.Y.) · 2011Article
- New mutant versions of yeast FACT subunit Spt16 affect cell integrity.Molecular genetics and genomics : MGG · 2009Article
- Article
- Initiation-mediated mRNA decay in yeast affects heat-shock mRNAs, and works through decapping and 5'-to-3' hydrolysis.Nucleic acids research · 2003Article
- Posttranscriptional control of gene expression in yeast.Microbiology and molecular biology reviews : MMBR · 1998Review
- Upf1 and Upf2 proteins mediate normal yeast mRNA degradation when translation initiation is limited.Nucleic acids research · 1998Article
- Fal1p is an essential DEAD-box protein involved in 40S-ribosomal-subunit biogenesis in Saccharomyces cerevisiae.Molecular and cellular biology · 1997Article
- Coupling of cell division to cell growth by translational control of the G1 cyclin CLN3 in yeast.Genes & development · 1997Article
- Glucose-dependent turnover of the mRNAs encoding succinate dehydrogenase peptides in Saccharomyces cerevisiae: sequence elements in the 5' untranslated region of the Ip mRNA play a dominant role.Molecular biology of the cell · 1995Article
- Mutational analysis of the Prt1 protein subunit of yeast translation initiation factor 3.Molecular and cellular biology · 1995Article
- Efficient translation of an SSA1-derived heat-shock mRNA in yeast cells limited for cap-binding protein and eIF-4F.Molecular & general genetics : MGG · 1995Article
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3 authors at 1 institution in 1 country.
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Abstract
The inhibition of translation initiation by modification or mutation of initiation factors can lead to disproportionate effects on gene expression. Here we report disproportionate decreases in gene expression in cells with mutated Prt1 activity. The PRT1 gene product of the budding yeast Saccharomyces cerevisiae is necessary for translation initiation and is thought to be a component of initiation factor 3. At a restrictive temperature the prt1-1 mutation, in addition to decreasing global protein synthesis, caused disproportionate decreases of the synthesis of the Ssa1 and Ssa2 members of the hsp70 heat-shock gene family, and of the Hsp82 and Hsc82 heat-shock proteins. Quantification of pulse-labelled, immunoprecipitated lacZ fusion proteins showed that synthesis of each of these proteins was disproportionately decreased in prt1-1 mutant cells. Although the mRNAs of affected genes were shown to be polysomal in mutant cells, they were fragmented and of decreased abundance, as indicated by transcript analysis and in vitro translation. Thus the mRNAs of these hsp genes become degraded under the conditions of limited translation initiation that are imposed by the prt1-1 mutation. This untimely mRNA degradation accounts for the disproportionate decreases in polypeptide synthesis in prt1 mutant cells. We propose that sequences at the translation initiation site of SSA2 mRNA bring about the observed mRNA fragmentation.
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