ArticleThe American journal of pathology1993
Cooperation of Ito cells and hepatocytes in the deposition of an extracellular matrix in vitro.
Article in The American journal of pathology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 99 citations in OpenAlex.
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- Norepinephrine-stimulated HSCs secrete sFRP1 to promote HCC progression following chronic stress via augmentation of a Wnt16B/β-catenin positive feedback loop.Journal of experimental & clinical cancer research : CR · 2020Article
- Hepatocyte-stellate cell cross-talk in the liver engenders a permissive inflammatory microenvironment that drives progression in hepatocellular carcinoma.Cancer research · 2012Article
- Co-culture of primary rat hepatocytes with rat liver epithelial cells enhances interleukin-6-induced acute-phase protein response.Cell and tissue research · 2010Article
- Perfused multiwell plate for 3D liver tissue engineering.Lab on a chip · 2010Article
- Liver tissue engineering in the evaluation of drug safety.Expert opinion on drug metabolism & toxicology · 2009Review
- Enhanced differentiation of embryonic stem cells using co-cultivation with hepatocytes.Biotechnology and bioengineering · 2008Article
- Regulation of matrix metallo-proteinase expression by extracellular matrix components in cultured hepatic stellate cells.Comparative hepatology · 2004Article
- Proliferation, functionality, and extracellular matrix production of hepatocytes and a liver stellate cell line: a comparison between single cultures and cocultures.Digestive diseases and sciences · 2003Article
- Effects of hypoxia, hyperoxia on the regulation of expression and activity of matrix metalloproteinase-2 in hepatic stellate cells.World journal of gastroenterology · 2001Article
- Expression of cellular prion protein in activated hepatic stellate cells.The American journal of pathology · 1998Article
- Differential expression and origin of membrane-type 1 and 2 matrix metalloproteinases (MT-MMPs) in association with MMP2 activation in injured human livers.The American journal of pathology · 1998Article
- Activation of matrix metalloproteinase-2 from hepatic stellate cells requires interactions with hepatocytes.The American journal of pathology · 1997Article
- Growth and differentiation in culture of clonogenic hepatocytes that express both phenotypes of hepatocytes and biliary epithelial cells.The American journal of pathology · 1996Article
- Serum type IV collagen in various liver diseases in comparison with serum 7S collagen, laminin, and type III procollagen peptide.Journal of gastroenterology · 1996Article
- Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes.The American journal of pathology · 1996Article
- Characterization and functional studies on rat liver fat-storing cell line and freshly isolated hepatocyte coculture system.The American journal of pathology · 1995Article
- Tissue distribution of liver regulating protein. Evidence for a cell recognition signal common to liver, pancreas, gonads, and hemopoietic tissues.The American journal of pathology · 1994Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular and molecular mechanisms involved in the deposition of extracellular matrix components in both normal and fibrotic liver are still poorly understood. We have investigated the influence of cooperation between Ito cells and hepatocytes in matrix deposition in vitro. Immunoprecipitation of radiolabeled proteins from media of 5-day-old Ito cell primary cultures showed that these cells secreted high levels of the major basement membrane components, ie, collagen IV, laminin, and entactin/nidogen. By immunocytochemistry, precursors of basement membrane components were found intracellularly, but only scarce deposits were seen around the cells. When hepatocytes were added to 2-day-old Ito cell primary cultures, they established close contacts with Ito cells in less than 24 hours and expressed ZO-1, a tight junction-associated protein not detectable in standard hepatocyte culture. Cytochemistry analysis revealed an abundant extracellular matrix deposited over hepatocyte cords and between hepatocytes and Ito cells. Immunocytochemistry studies showed that this matrix contained laminin, fibronectin, and collagens proIII and IV. These data indicate that a high level of matrix protein synthesis by liver cells in vitro is not sufficient to induce extracellular matrix deposition, and that cell-cell interactions are strongly involved in this process. Hepatocyte/Ito cell co-culture, which may reflect the actual situation in vivo, represents a useful tool for studying liver fibrogenesis.
Indexed as
Identifiers
8342601PMC1887016W243649834What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.