ArticleProceedings of the National Academy of Sciences of the United States of America1993
Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Anaphase B.Biology · 2016Review
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Mitotic force generators and chromosome segregation.Cellular and molecular life sciences : CMLS · 2010Review
- Microtubules in Candida albicans hyphae drive nuclear dynamics and connect cell cycle progression to morphogenesis.Eukaryotic cell · 2005Article
- Model for anaphase B: role of three mitotic motors in a switch from poleward flux to spindle elongation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Identification and characterization of a novel microtubule-based motor associated with membranous organelles in tobacco pollen tubes.The Plant cell · 2000Article
- Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4.Molecular biology of the cell · 1998Article
- CDK1 inactivation regulates anaphase spindle dynamics and cytokinesis in vivo.The Journal of cell biology · 1997Article
- Anaphase in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function.Molecular biology of the cell · 1996Article
- DSK1, a novel kinesin-related protein from the diatom Cylindrotheca fusiformis that is involved in anaphase spindle elongation.The Journal of cell biology · 1996Article
- Going mobile: microtubule motors and chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- A bipolar kinesin.Nature · 1996Article
- A kinesin-like protein, KatAp, in the cells of arabidopsis and other plants.The Plant cell · 1996Article
- Identification of the chromosome localization domain of the Drosophila nod kinesin-like protein.The Journal of cell biology · 1995Article
- The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis.The Journal of cell biology · 1995Article
- Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.The Journal of cell biology · 1995Article
- Molecular cloning and characterization of human kinectin.Molecular biology of the cell · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated central spindles or spindles in detergent-permeabilized cells from the diatom Cylindrotheca fusiformis can undergo ATP-dependent reactivation of spindle elongation in vitro. We have used a peptide antibody raised against a 10-amino acid portion common to the kinesin superfamily motor domain to look for kinesin-like motor activity during anaphase B of mitosis. The peptide antibody localizes to central spindles. Upon ATP reactivation of spindle elongation, antigens recognized by the antibody are associated exclusively with the central spindle midzone where antiparallel microtubules of each half-spindle overlap. The antibody recognizes several polypeptides by immunoblot using isolated spindle extracts. One of these polypeptides behaves like kinesin with respect to nucleotide-specific binding to and release from taxol-stabilized microtubules. Preincubation of the spindle model with the peptide antibody inhibits subsequent ATP reactivation of spindle elongation. Coincubation of the peptide antibody with peptide antigen rescues spindle function. These results support a role for kinesin-related protein(s) in spindle elongation (anaphase B) of mitosis and suggest that one or several polypeptides that we have identified in spindle extracts may fulfill this function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.