ArticleProceedings of the National Academy of Sciences of the United States of America1993
Expression of an atrial G-protein-activated potassium channel in Xenopus oocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 84 citations in OpenAlex.
- PI(4,5)PAdvances in experimental medicine and biology · 2023Article
- Sodium ions allosterically modulate the M2 muscarinic receptor.Scientific reports · 2020Article
- Mutual action by Gγ and Gβ for optimal activation of GIRK channels in a channel subunit-specific manner.Scientific reports · 2019Article
- Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor.Nature communications · 2017Article
- Differential Desensitization Observed at Multiple Effectors of Somatic μ-Opioid Receptors Underlies Sustained Agonist-Mediated Inhibition of Proopiomelanocortin Neuron Activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- A Quantitative Model of the GIRK1/2 Channel Reveals That Its Basal and Evoked Activities Are Controlled by Unequal Stoichiometry of Gα and Gβγ.PLoS computational biology · 2015Article
- G protein-coupled receptor signaling to Kir channels in Xenopus oocytes.Current pharmaceutical biotechnology · 2014Review
- Gating of a G protein-sensitive mammalian Kir3.1 prokaryotic Kir channel chimera in planar lipid bilayers.The Journal of biological chemistry · 2010Article
- Do caveolae have a role in the fidelity and dynamics of receptor activation of G-protein-gated inwardly rectifying potassium channels?The Journal of biological chemistry · 2010Article
- Physical determinants of strong voltage sensitivity of K(+) channel block.Nature structural & molecular biology · 2009Article
- Engineered specific and high-affinity inhibitor for a subtype of inward-rectifier K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Cell signal control of the G protein-gated potassium channel and its subcellular localization.The Journal of physiology · 2004Review
- Mechanism of rectification in inward-rectifier K+ channels.The Journal of general physiology · 2003Article
- Brain localization and behavioral impact of the G-protein-gated K+ channel subunit GIRK4.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- The recombinant 5-HT1A receptor: G protein coupling and signalling pathways.British journal of pharmacology · 1999Review
- Isoprenaline can activate the acetylcholine-induced K+ current in canine atrial myocytes via Gs-derived betagamma subunits.The Journal of physiology · 1999Article
- Voltage-dependent gating of single wild-type and S4 mutant KAT1 inward rectifier potassium channels.The Journal of general physiology · 1998Article
- Positive and negative coupling of the metabotropic glutamate receptors to a G protein-activated K+ channel, GIRK, in Xenopus oocytes.The Journal of general physiology · 1997Article
- The structure and organization of synthetic putative membranous segments of ROMK1 channel in phospholipid membranes.Biophysical journal · 1997Article
- Metabotropic glutamate receptors activate G-protein-coupled inwardly rectifying potassium channels in Xenopus oocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Injection of rat atrial RNA into Xenopus oocytes resulted in the expression of guanine nucleotide binding (G) protein-activated K+ channel. Current through the channel could be activated by acetylcholine or, if RNA encoding a neuronal 5HT1A receptor was coinjected with atrial RNA, by serotonin (5HT). A 5HT-evoked current (I5HT) was observed in oocytes injected with ventricle RNA fractions (of 2.5-5.5 kb) and 5HT1A receptor RNA. I5HT displayed strong inward rectification with very little conductance above the K+ equilibrium potential, was highly selective for K+ over Na+, and was blocked by 5-300 microM Ba2+. I5HT was suppressed by intracellular injection of the nonhydrolyzable analog of GDP, guanosine 5'-[beta-thio]diphosphate, but not by treatment with pertussis toxin (PTX), suggesting coupling of the receptor to the G-protein-activated K+ channel via a PTX-insensitive G protein, possibly endogenously present in the oocyte. Coexpression of the alpha subunit of a PTX-sensitive G protein, G(i2), rendered I5HT sensitive to PTX inhibition. Native oocytes displayed a constitutively active inwardly rectifying K+ current with a lower sensitivity to Ba2+ block; expression of a similar current was also directed by atrial or ventricle RNA of 1.5-3 kb. Xenopus oocytes may be employed for cloning of the G-protein-activated K+ channel cDNA and for studying the coupling between this channel and G proteins.
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