ArticleNucleic acids research1993
Interactions of USF and Ku antigen with a human DNA region containing a replication origin.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.Scientific reports · 2019Article
- DNA-protein interaction dynamics at the Lamin B2 replication origin.Cell cycle (Georgetown, Tex.) · 2015Article
- The human stress-activated protein kin17 belongs to the multiprotein DNA replication complex and associates in vivo with mammalian replication origins.Molecular and cellular biology · 2005Article
- Involvement of cellular double-stranded DNA break binding proteins in processing of the recombinant adeno-associated virus genome.Journal of virology · 2001Article
- In vivo association of Ku with mammalian origins of DNA replication.Molecular biology of the cell · 2001Article
- DNA replication efficiency depends on transcription factor-binding sites.Journal of virology · 2001Article
- OBA/Ku86: DNA binding specificity and involvement in mammalian DNA replication.Molecular biology of the cell · 1999Article
- Cell cycle modulation of protein-DNA interactions at a human replication origin.The EMBO journal · 1998Article
- Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.Nucleic acids research · 1998Review
- In vivo protein-DNA interactions at human DNA replication origin.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Purification and properties of human DNA helicase VI.Nucleic acids research · 1995Article
- Molecular and functional interactions of transcription factor USF with the long terminal repeat of human immunodeficiency virus type 1.Journal of virology · 1995Article
- Stimulation of the adenovirus major late promoter in vitro by transcription factor USF is enhanced by the adenovirus DNA binding protein.Journal of virology · 1994Article
- Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen.The EMBO journal · 1994Article
- A novel DNA replication origin identified in the human heat shock protein 70 gene promoter.Molecular and cellular biology · 1994Article
- Fine mapping of a replication origin of human DNA.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- The quest for a human ori.Genetica · 1994Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By means of a combination of ion-exchange and sequence-specific affinity chromatography techniques, we have purified to homogeneity two protein complexes binding in a human DNA region (B48) previously recognized to contain a DNA replication origin. The DNA sequence used for the protein purification (B48 binding site) contains a binding site for basic-helix-loop-helix DNA binding proteins. The first complex is composed of two polypeptides of 42- and 44-kDa; its size, heat stability, and target DNA sequence suggest that it corresponds to transcription factor USF; furthermore, the 42-kDa polypeptide is recognized by antibodies raised against 43-kDa-USF. The second complex is represented by equimolar amounts of two proteins of 72 and 87 kDa; microsequencing of the two species indicated that they correspond to the human Ku antigen. In analogy with Ku, they produce a regular pattern of footprints without an apparent sequence-specificity, and their binding can be competed by unspecific DNA provided that it contains free ends. The potential role of B48 binding site and of these cognate proteins in origin activation is discussed.
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