ArticleMolecular and cellular biology1993
Tissue-specific alternative splicing of the Drosophila dopa decarboxylase gene is affected by heat shock.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins modulates splicing.Nucleic acids research · 2009Article
- Stress and the cell nucleus: dynamics of gene expression and structural reorganization.Gene expression · 1999Review
- Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme.Neurochemical research · 1996Review
- Altered levels of the Drosophila HRB87F/hrp36 hnRNP protein have limited effects on alternative splicing in vivo.Molecular biology of the cell · 1996Article
- Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The SR protein B52/SRp55 is essential for Drosophila development.Molecular and cellular biology · 1994Article
- cis-regulatory sequences responsible for alternative splicing of the Drosophila dopa decarboxylase gene.Molecular and cellular biology · 1994Article
- Alternative 5' splice site selection induced by heat shock.Molecular and cellular biology · 1994Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Drosophila dopa decarboxylase gene, Ddc, is expressed in the hypoderm and in a small number of cells in the central nervous system (CNS). The unique Ddc primary transcript is alternatively spliced in these two tissues. We investigated whether Ddc splicing in the CNS is a general property of the CNS or a unique property of the cells that normally express Ddc by expressing the Ddc primary transcript ubiquitously under the control of an Hsp70 heat shock promoter. Under basal expression conditions, Ddc splicing shows normal tissue specificity, indicating that the regulation of Ddc splicing in the CNS is tissue specific rather than cell specific. Previous studies have shown that severe heat shock blocks mRNA splicing in cultured Drosophila melanogaster cells. Our results show that splicing of the heat shock-inducible Hsp83 transcript is very resistant to heat shock. In contrast, under either mild or severe heat shock, the splicing specificity of the heat shock-induced Ddc primary transcript is affected, leading to the accumulation of inappropriately high levels of the CNS splice form in non-CNS tissues. The chromosomal Ddc transcript is similarly affected. These results show unexpected heterogeneity in the splicing of individual mRNAs as a response to heat shock and suggest that the Ddc CNS-specific splicing pathway is the default.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.