ArticleThe Journal of cell biology1993
Calcium-calmodulin and regulation of brush border myosin-I MgATPase and mechanochemistry.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 86 citations in OpenAlex.
- The dynamics of actin protrusions can be controlled by tip-localized myosin motors.The Journal of biological chemistry · 2024Article
- Manipulation of microvillar proteins duringFrontiers in cellular and infection microbiology · 2023Article
- Molecular analysis of CIB4 gene and protein in Kermani sheep.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Various Themes of Myosin Regulation.Journal of molecular biology · 2016Review
- Shaping the intestinal brush border.The Journal of cell biology · 2014Review
- Membrane-bound myo1c powers asymmetric motility of actin filaments.Current biology : CB · 2012Article
- Leveraging the membrane - cytoskeleton interface with myosin-1.Trends in cell biology · 2010Review
- Myosin-1a powers the sliding of apical membrane along microvillar actin bundles.The Journal of cell biology · 2007Article
- The Dictyostelium class I myosin, MyoD, contains a novel light chain that lacks high-affinity calcium-binding sites.The Biochemical journal · 2003Article
- Regulatory implications of a novel mode of interaction of calmodulin with a double IQ-motif target sequence from murine dilute myosin V.Protein science : a publication of the Protein Society · 2002Article
- MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.Biophysical journal · 2002Article
- Myosin VIIA is specifically associated with calmodulin and microtubule-associated protein-2B (MAP-2B).The Biochemical journal · 2001Article
- Inhibitory regulation of higher-plant myosin by Ca2+ ionsPlant physiology · 1999Article
- Three-dimensional structure of Acanthamoeba castellanii myosin-IB (MIB) determined by cryoelectron microscopy of decorated actin filaments.The Journal of cell biology · 1998Article
- Distinct functions of calmodulin are required for the uptake step of receptor-mediated endocytosis in yeast: the type I myosin Myo5p is one of the calmodulin targets.The EMBO journal · 1998Article
- Kinetic characterization of brush border myosin-I ATPase.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Brush border myosin-I structure and ADP-dependent conformational changes revealed by cryoelectron microscopy and image analysis.The Journal of cell biology · 1997Article
- In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- ATPase activity of myosin in hair bundles of the bullfrog's sacculus.Biophysical journal · 1997Article
- A myosin family reunion.Journal of muscle research and cell motility · 1996Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We examined the Ca(2+)-dependent regulation of brush border (BB) myosin-I by probing the possible roles of the calmodulin (CM) light chains. BB myosin-I MgATPase activity, sensitivity to chymotryptic digestion, and mechanochemical properties were assessed using 1-10 microM Ca2+ and in the presence of exogenously added CM since it has been proposed that this myosin is regulated by calcium-induced CM dissociation from the 119-kD heavy chain. Each of these BB myosin-I properties were dramatically altered by the same threshold of 2-3 microM Ca2+. Enzymatically active NH2-terminal proteolytic fragments of BB myosin-I which lack the CM binding domains (the 78-kD peptide) differ from CM-containing peptides in that the former is completely insensitive to Ca2+. Furthermore, the 78-kD peptide exhibits high levels of MgATPase activity which are comparable to that observed for BB myosin-I in the presence of Ca2+. This suggests that Ca2+ regulates BB myosin-I MgATPase by binding directly to the CM light chains, and that CM acts to repress endogenous MgATPase activity. Ca(2+)-induced CM dissociation from BB myosin-I can be prevented by the addition of exogenous CM. Under these conditions Ca2+ causes a reversible slowing of motility. In contrast, in the absence of exogenous CM, motility is stopped by Ca2+. We demonstrate this reversible slowing is not due to the presence of inactive BB myosin-I molecules exerting a "braking" effect on motile filaments. However, we did observe Ca(2+)-independent slowing of motility by acidic phospholipids, suggesting that factors other than Ca2+ and CM content can affect the mechanochemical properties of BB myosin-I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.