ArticleThe EMBO journal1993
An E2F-binding site mediates cell-cycle regulated repression of mouse B-myb transcription.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 139 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
139 citing papers in PubMed, 340 citations in OpenAlex.
- The ICF syndrome protein CDCA7 harbors a unique DNA binding domain that recognizes a CpG dyad in the context of a non-B DNA.Science advances · 2024Article
- MYB oncoproteins: emerging players and potential therapeutic targets in human cancer.Oncogenesis · 2021Review
- Association between B-Oncology letters · 2021Review
- Golgi OncoproteinGenes · 2019Article
- Structural mechanism of Myb-MuvB assembly.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Wild-type Kras expands and exhausts hematopoietic stem cells.JCI insight · 2018Article
- TheBiology open · 2017Article
- Article
- Article
- Cellular senescence and aging: the role of B-MYB.Aging cell · 2014Review
- Article
- B-myb is an essential regulator of hematopoietic stem cell and myeloid progenitor cell development.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1).Cell cycle (Georgetown, Tex.) · 2012Article
- 58-kDa microspherule protein (MSP58) is novel Brahma-related gene 1 (BRG1)-associated protein that modulates p53/p21 senescence pathway.The Journal of biological chemistry · 2012Article
- The CHR promoter element controls cell cycle-dependent gene transcription and binds the DREAM and MMB complexes.Nucleic acids research · 2012Article
- Mybl2, downregulated during colon epithelial cell maturation, is suppressed by miR-365.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Addiction of MYCN amplified tumours to B-MYB underscores a reciprocal regulatory loop.Oncotarget · 2010Article
- E2F-1 has dual roles depending on the cell cycle.International journal of biological sciences · 2010Article
- Enhanced proliferation of monolayer cultures of embryonic stem (ES) cell-derived cardiomyocytes following acute loss of retinoblastoma.PloS one · 2008Article
- Targeting an E2F site in the mouse genome prevents promoter silencing in quiescent and post-mitotic cells.Oncogene · 2007Article
79 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the B-myb gene is regulated at the G1/S boundary of the cell cycle. To begin to examine the mechanism controlling expression of this gene during the cell-cycle, a mouse B-myb 5' flanking sequence was isolated from a cosmid library and shown to promote efficiently the transcription of a luciferase reporter gene when transfected into NIH3T3 fibroblasts. It was further shown that in transfected cells released from G0 by readdition of serum, luciferase activity directed by the B-myb promoter was induced substantially as cells entered S phase, thus paralleling the regulation of endogenous B-myb. Analysis of the B-myb promoter identified a region that appeared to have no intrinsic promoter activity yet which acted to regulate transcription negatively in G0. Mutagenesis of an E2F consensus binding site within this region was sufficient to relieve transcription repression in G0, resulting in a promoter with constitutively high activity. Specific G0 and S phase E2F complexes binding to this B-myb element were detected in NIH3T3 cell extracts by mobility shift assays. These studies demonstrate for the first time a direct role for E2F in regulation of cell cycle gene expression by repression of transcription in G0/early G1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.