Evidence map›Paper›PMID 8328833›Full record

ArticleAnnals of surgery1993

Comparison of peripheral blood leukocyte kinetics after live Escherichia coli, endotoxin, or interleukin-1 alpha administration. Studies using a novel interleukin-1 receptor antagonist.

A S Hawes, E Fischer, M A Marano, K J Van Zee, C S Rock, S F Lowry, S E Calvano, L L Moldawer

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of surgery, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

A S HawesDepartment of Surgery, Cornell University Medical College, New York, New York.
E Fischer
M A Marano
K J Van Zee
C S Rock
S F Lowry
S E Calvano
L L Moldawer
Cornell University · US

Funding

HORMONE AND CYTOKINE REGULATION OF ENDOTOXIN INJURY(RMI)R37GM034695 · NIGMS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI LOWRY, STEPHEN F · 1997 to 2006
$4.0M
SKELETAL MUSCLE PROTEIN RESPONSE TO INJURY AND REPLETIONR01GM034695 · NIGMS · WEILL MEDICAL COLLEGE OF CORNELL UNIV · PI CORBETT, SIOBHAN A · 1985 to 2011
$2.8M
REGULATION ON MONOKINE PRODUCTION IN TRAUMA AND SURGERYR01GM040586 · NIGMS · WEILL MEDICAL COLLEGE OF CORNELL UNIV · PI MOLDAWER, LYLE L · 1988 to 2016
$2.6M
CYTOKINE REGULATION IN SEPSIS/INFLAMMATIONR37GM040586 · NIGMS · UNIVERSITY OF FLORIDA · PI MOLDAWER, LYLE L · 1998 to 2007
$2.3M
CYTOKINES AS ENDOGENOUS MEDIATORS OF CANCER CACHEXIAR01CA052108 · NCI · WEILL MEDICAL COLLEGE OF CORNELL UNIV · PI MOLDAWER, LYLE L · 1990 to 1994
–
NCI NIH HHS CA 52108NIGMS NIH HHS GM 34695NIGMS NIH HHS GM 40586
6 · The paper itself

Abstract

objectiveThis study was undertaken to evaluate whether hematologic and immunologic effects observed after bacteremia and endotoxemia in the host could be replicated by administration of recombinant human interleukin-1 alpha (IL-1 alpha) in a primate model. Furthermore, to determine whether endogenously produced interleukin-1 (IL-1) contributes to the changes observed during endotoxemia or gram-negative septic shock, a specific IL-1 receptor antagonist (IL-1 ra) was administered. SUMMARY BACKGROUND DATA: The lipopolysaccharide (LPS) component of the outer membrane of gram-negative bacteria initiates a constellation of metabolic and immunologic host responses. IL-1, a macrophage-derived cytokine, acts as a key mediator in the host response to infection and inflammation.

methodsBaboons were randomly assigned to receive either recombinant human IL-1 alpha, LPS, or live Escherichia coli both with or without concomitant administration of IL-1ra. Blood was collected hourly and analyzed using flow cytometric techniques.

resultsBoth endotoxemia and live E. coli bacteremia induced an acute granulocytopenia; however, the granulocytopenia gradually resolved in the endotoxemic group, but was sustained in the bacteremic group. An early lymphopenia and monocytopenia was elicited by LPS or E. coli and persisted throughout the experiment. Recombinant human IL-1 alpha induced the following: (1) an early, transient decline in granulocytes followed by a sustained granulocytosis; (2) a lymphopenia; and (3) a transient monocytopenia followed by a gradual return to baseline. Although IL-1ra had no effect on leukocyte kinetics with either live E. coli or LPS, the IL-1ra significantly abrogated the monocytopenia seen with recombinant human IL-1 alpha administration alone.

conclusionsThese results suggest that administration of recombinant human IL-1 alpha can replicate some of the characteristic patterns of hematologic change associated with bacteremia and endotoxemia. However, an endogenous IL-1 response is not required for these changes to occur. Rather, the data suggest that other inflammatory mediators induced by endotoxemia or gram-negative bacteremia, such as tumor necrosis factor-alpha (TNF alpha), may be involved.

Indexed as

AnimalsBacteremiaCell DivisionEndotoxinsEscherichia coliFemaleGranulocytesInterleukin-1Interleukin 1 Receptor Antagonist ProteinKineticsLeukocytesLipopolysaccharidesMaleMonocytesPapioRandom AllocationEndotoxinsIL1RN protein, humanInterleukin-1Interleukin 1 Receptor Antagonist ProteinLipopolysaccharidesSialoglycoproteins

Identifiers

PMID8328833
PMCPMC1242904
OpenAlexW2039627131

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.