ArticleBiophysical journal1993
Viscosity of passive human neutrophils undergoing small deformations.
Article in Biophysical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 131 citations in OpenAlex.
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Mechanical characterization of vesicles and cells: A review.Electrophoresis · 2020Review
- Characterization of cytoplasmic viscosity of hundreds of single tumour cells based on micropipette aspiration.Royal Society open science · 2019Article
- Review
- Techniques to stimulate and interrogate cell-cell adhesion mechanics.Extreme Mechanics Letters · 2018Article
- Controlled One-on-One Encounters between Immune Cells and Microbes Reveal Mechanisms of Phagocytosis.Biophysical journal · 2015Review
- A mechanism for effective cell-seeding in rigid, microporous substrates.Acta biomaterialia · 2013Article
- Numerical simulation of passage of a neutrophil through a rectangular channel with a moderate constriction.PloS one · 2013Article
- Leukocyte rolling on P-selectin: a three-dimensional numerical study of the effect of cytoplasmic viscosity.Biophysical journal · 2012Article
- Article
- Mechanical forces induced by the transendothelial migration of human neutrophils.Biophysical journal · 2008Article
- Rheology of passive and adhesion-activated neutrophils probed by atomic force microscopy.Biophysical journal · 2006Article
- A 3-D computational model predicts that cell deformation affects selectin-mediated leukocyte rolling.Biophysical journal · 2005Article
- The mechanics of neutrophils: synthetic modeling of three experiments.Biophysical journal · 2003Article
- Neutrophil transit times through pulmonary capillaries: the effects of capillary geometry and fMLP-stimulation.Biophysical journal · 2002Article
- Aspiration of human neutrophils: effects of shear thinning and cortical dissipation.Biophysical journal · 2001Article
- Cell sorting is analogous to phase ordering in fluids.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- An automatic braking system that stabilizes leukocyte rolling by an increase in selectin bond number with shear.The Journal of cell biology · 1999Article
- Hydrodynamics of micropipette aspiration.Biophysical journal · 1999Article
- Viscoelastic properties of living embryonic tissues: a quantitative study.Biophysical journal · 1998Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
At issue is the type of constitutive equation that can be used to describe all possible types of deformation of the neutrophil. Here a neutrophil undergoing small deformations is studied by aspirating it into a glass pipet with a diameter that is only slightly smaller than the diameter of the spherically shaped cell. After being held in the pipet for at least seven seconds, the cell is rapidly expelled and allowed to recover its undeformed, spherical shape. The recovery takes approximately 15 s. An analysis of the recovery process that treats the cell as a simple Newtonian liquid drop with a constant cortical (surface) tension gives a value of 3.3 x 10(-5) cm/s for the ratio of the cortical tension to cytoplasmic viscosity. This value is about twice as large as a previously published value obtained with the same model from studies of large deformations of neutrophils. This discrepancy indicates that the cytoplasmic viscosity decreases as the amount of deformation decreases. An extrapolated value for the cytoplasmic viscosity at zero deformation is approximately 600 poise when a value for the cortical tension of 0.024 dyn/cm is assumed. Clearly the neutrophil does not behave like a simple Newtonian liquid drop in that small deformations are inherently different from large deformations. More complex models consisting either of two or more fluids or multiple shells must be developed. The complex structure inside the neutrophil is shown in scanning electron micrographs of osmotically burst cells and cells whose membrane has been dissolved away.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.