ArticleMolecular and cellular biology1993
Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 56 citations in OpenAlex.
- A lifespan atlas of the killifish testis defines a pronounced mid-age remodeling shift during testicular aging.BMC biology · 2026Article
- TSSK3, a novel target for male contraception, is required for spermiogenesis.Molecular reproduction and development · 2021Article
- Distinct expression patterns of ICK/MAK/MOK protein kinases in the intestine implicate functional diversity.PloS one · 2013Article
- Mitogen-activated protein kinase signaling in plant-interacting fungi: distinct messages from conserved messengers.The Plant cell · 2012Review
- Exome sequencing and analysis of induced pluripotent stem cells identify the cilia-related gene male germ cell-associated kinase (MAK) as a cause of retinitis pigmentosa.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Ime1 and Ime2 are required for pseudohyphal growth of Saccharomyces cerevisiae on nonfermentable carbon sources.Molecular and cellular biology · 2010Article
- The promoter for intestinal cell kinase is head-to-head with F-Box 9 and contains functional sites for TCF7L2 and FOXA factors.Molecular cancer · 2010Article
- Intestinal cell kinase, a MAP kinase-related kinase, regulates proliferation and G1 cell cycle progression of intestinal epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Tssk6 is required for Izumo relocalization and gamete fusion in the mouse.Journal of cell science · 2009Article
- Molecular cloning and expression profile analysis of a novel mouse testis-specific expression gene mtIQ1.Molecular biology reports · 2009Article
- The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae.Genetics · 2009Article
- Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase.Molecular and cellular biology · 2006Article
- The induction of sexual development and virulence in the smut fungus Ustilago maydis depends on Crk1, a novel MAPK protein.Genes & development · 2004Article
- Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2.Genes & development · 2003Article
- A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.Molecular and cellular biology · 2002Article
- A novel family of serine/threonine kinases participating in spermiogenesis.The Journal of cell biology · 1997Article
- A bipartite operator interacts with a heat shock element to mediate early meiotic induction of Saccharomyces cerevisiae HSP82.Molecular and cellular biology · 1995Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mak gene encodes a new protein kinase distantly related to cdc2 kinase, and its transcripts are expressed exclusively in testicular germ cells at and after meiosis (H. Matsushime, A. Jinno, N. Takagi, and M. Shibuya, Mol. Cell. Biol. 10:2261-2268, 1990). In this study, we prepared a series of antibodies against synthetic peptides and fusion products of the mak gene and characterized the subcellular localization, protein kinase activity, and association with other cellular proteins of Mak. Mak products were identified as 66- and 60-kDa proteins that specifically appeared in rat testes after puberty. Testicular germ cell fractionation revealed that Mak products were most abundant in the fraction of the late pachytene stage and that their levels were dramatically decreased in postmeiotic haploid cells. Mak products were localized mostly in the cytoplasm as a soluble form. [35S]methionine labelling demonstrated that Mak products were associated with a 210-kDa cellular protein; in an in vitro kinase assay with immunoprecipitates of Mak products, the 210-kDa cellular protein was efficiently phosphorylated on serine and threonine residues. Furthermore, in a testicular cell culture system with 32Pi, the 210-kDa molecule associated with Mak was phosphorylated in vivo on serine and threonine residues. These results strongly suggest that the Mak complex may play a role in meiosis during spermatogenesis and that a phosphorylated 210-kDa protein is one of the physiological substrates for this protein kinase.
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