ArticleMolecular and cellular biology1993
The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 105 citations in OpenAlex.
- Multilayered control of hexose uptake and phosphorylation through specialized sugar kinases in Nakaseomyces glabratus.Fungal biology and biotechnology · 2026Article
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.PLoS genetics · 2023Article
- Genetic and Physiological Characterization of Fructose-1,6-Bisphosphate Aldolase and Glyceraldehyde-3-Phosphate Dehydrogenase in the Crabtree-Negative YeastInternational journal of molecular sciences · 2022Article
- Moonlighting Proteins: The Case of the Hexokinases.Frontiers in molecular biosciences · 2021Review
- Article
- Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses.Biotechnology for biofuels · 2015Article
- Proteomic and functional consequences of hexokinase deficiency in glucose-repressible Kluyveromyces lactis.Molecular & cellular proteomics : MCP · 2014Article
- Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- The SWI/SNF KlSnf2 subunit controls the glucose signaling pathway to coordinate glycolysis and glucose transport in Kluyveromyces lactis.Eukaryotic cell · 2012Article
- Crystal structure of hexokinase KlHxk1 of Kluyveromyces lactis: a molecular basis for understanding the control of yeast hexokinase functions via covalent modification and oligomerization.The Journal of biological chemistry · 2010Article
- Characterization of KlGRR1 and SMS1 genes, two new elements of the glucose signaling pathway of Kluyveromyces lactis.Eukaryotic cell · 2008Article
- Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes.Current genetics · 2007Article
- Connection between the Rag4 glucose sensor and the KlRgt1 repressor in Kluyveromyces lactis.Genetics · 2006Article
- Enolase and glycolytic flux play a role in the regulation of the glucose permease gene RAG1 of Kluyveromyces lactis.Genetics · 2004Article
- Functional characterisation and transcriptional regulation of the KlHEM12 gene from Kluyveromyces lactis.Current genetics · 2004Article
- Improved production of heterologous proteins by a glucose repression-defective mutant of Kluyveromyces lactis.Applied and environmental microbiology · 2004Article
- Regulation of glycolysis in Kluyveromyces lactis: role of KlGCR1 and KlGCR2 in glucose uptake and catabolism.Current genetics · 2004Article
- KNQ1, a Kluyveromyces lactis gene encoding a drug efflux permease.Current genetics · 2004Article
- Novel DNA-binding proteins in the cyanobacterium Anabaena sp. strain PCC 7120.Journal of bacteriology · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RAG1 gene of Kluyveromyces lactis encodes a low-affinity glucose/fructose transporter. Its transcription is induced by glucose, fructose, and several other sugars. The RAG4, RAG5, and RAG8 genes are trans-acting genes controlling the expression of the RAG1 gene. We report here the characterization of one of these genes, RAG5. The nucleotide sequence of the cloned RAG5 gene indicated that it encodes a protein that is homologous to hexokinases of Saccharomyces cerevisiae. rag5 mutants showed no detectable hexokinase or glucokinase activity, suggesting that the sugar kinase activity encoded by this gene is the only hexokinase in K. lactis. Both high- and low-affinity transport systems of glucose were affected in rag5 mutants. The defect of the low-affinity component was found to be due to a block of transcription of the RAG1 gene by the hexokinase mutation. In vivo complementation of the rag5 mutation by the HXK2 gene of S. cerevisiae and complementation of hxk1 hxk2 mutations of S. cerevisiae by the RAG5 gene showed that RAG5 and HXK2 were equivalent for sugar-phosphorylating activity but that RAG5 could not restore glucose repression in the S. cerevisiae hexokinase mutants.
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