ArticleThe Journal of cell biology1993
Microtubule nucleation and release from the neuronal centrosome.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Polarity reversal of stable microtubules during neuronal development.Journal of cell science · 2025Article
- Article
- Measurements of neurite extension and nucleokinesis in an iPSC-derived model system following microtubule perturbation.Molecular biology of the cell · 2025Article
- Proteasome localization and activity in pig brain andFrontiers in cellular neuroscience · 2024Article
- Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton.Frontiers in cell and developmental biology · 2023Review
- Review
- To nucleate or not, that is the question in neurons.Neuroscience letters · 2021Review
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- The nano-architecture of the axonal cytoskeleton.Nature reviews. Neuroscience · 2017Review
- Microtubule organization, dynamics and functions in differentiated cells.Development (Cambridge, England) · 2017Review
- Of microtubules and memory: implications for microtubule dynamics in dendrites and spines.Molecular biology of the cell · 2017Review
- Microtubules in health and degenerative disease of the nervous system.Brain research bulletin · 2016Review
- Stability properties of neuronal microtubules.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.Nature communications · 2016Article
- Loss of γ-tubulin, GCP-WD/NEDD1 and CDK5RAP2 from the Centrosome of Neurons in Developing Mouse Cerebral and Cerebellar Cortex.Acta histochemica et cytochemica · 2015Article
- Γ-tubulin controls neuronal microtubule polarity independently of Golgi outposts.Molecular biology of the cell · 2014Article
- Article
- Microtubule dynamics in neuronal morphogenesis.Open biology · 2013Review
- A novel MAPT mutation, G55R, in a frontotemporal dementia patient leads to altered Tau function.PloS one · 2013Article
- Golgi outposts shape dendrite morphology by functioning as sites of acentrosomal microtubule nucleation in neurons.Neuron · 2012Article
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4 authors.
Funding
Abstract
We have proposed that microtubules (MTs) destined for axons and dendrites are nucleated at the centrosome within the cell body of the neuron, and are then released for translocation into these neurites (Baas, P. W., and H. C. Joshi. 1992. J. Cell Biol. 119:171-178). In the present study, we have tested the capacity of the neuronal centrosome to act as a generator of MTs for relocation into other regions of the neuron. In cultured sympathetic neurons undergoing active axonal outgrowth, MTs are present throughout the cell body including the region around the centrosome, but very few (< 10) are directly attached to the centrosome. These results indicate either that the neuronal centrosome is relatively inactive with regard to MT nucleation, or that most of the MTs nucleated at the centrosome are rapidly released. Treatment for 6 h with 10 micrograms/ml nocodazole results in the depolymerization of greater than 97% of the MT polymer in the cell body. Within 5 min after removal of the drug, hundreds of MTs have assembled in the region of the centrosome, and most of these MTs are clearly attached to the centrosome. A portion of the MTs are not attached to the centrosome, but are aligned side-by-side with the attached MTs, suggesting that the unattached MTs were released from the centrosome after nucleation. In addition, unattached MTs are present in the cell body at decreasing levels with increasing distance from the centrosome. By 30 min, the MT array of the cell body is indistinguishable from that of controls. The number of MTs attached to the centrosome is once again diminished to fewer than 10, suggesting that the hundreds of MTs nucleated from the centrosome after 5 min were subsequently released and translocated away from the centrosome. These results indicate that the neuronal centrosome is a highly potent MT-nucleating structure, and provide strong indirect evidence that MTs nucleated from the centrosome are released for translocation into other regions of the neuron.
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