ArticleThe Journal of cell biology1993
The cytoskeletal protein talin contains at least two distinct vinculin binding domains.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 74 citations in OpenAlex.
- Talin-tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation.The Journal of cell biology · 2026Article
- Patient genetics is linked to chronic wound microbiome composition and healing.PLoS pathogens · 2020Article
- α-actinin is required for the proper assembly of Z-disk/focal-adhesion-like structures and for efficient locomotion in Caenorhabditis elegans.Journal of molecular biology · 2010Article
- Structural determinants of integrin binding to the talin rod.The Journal of biological chemistry · 2009Article
- Further characterization of the interaction between the cytoskeletal proteins talin and vinculin.The Biochemical journal · 2002Article
- Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix.The Biochemical journal · 1999Article
- Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles.The Journal of cell biology · 1998Article
- Disruption of the talin gene compromises focal adhesion assembly in undifferentiated but not differentiated embryonic stem cells.The Journal of cell biology · 1998Article
- Vinculin is part of the cadherin-catenin junctional complex: complex formation between alpha-catenin and vinculin.The Journal of cell biology · 1998Article
- Isolation of peptides from phage-displayed random peptide libraries that interact with the talin-binding domain of vinculin.The Biochemical journal · 1997Article
- Talin and vinculin play distinct roles in filopodial motility in the neuronal growth cone.The Journal of cell biology · 1996Article
- Talin requires beta-integrin, but not vinculin, for its assembly into focal adhesion-like structures in the nematode Caenorhabditis elegans.Molecular biology of the cell · 1996Article
- Vinculin, talin and focal adhesions.Journal of muscle research and cell motility · 1996Review
- Localisation of the human gene encoding the cytoskeletal protein talin to chromosome 9p.Human genetics · 1995Article
- Interaction of the 47-kDa talin fragment and the 32-kDa vinculin fragment with acidic phospholipids: a computer analysis.Biophysical journal · 1995Article
- A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.The Journal of cell biology · 1995Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have mapped the vinculin-binding sites in the cytoskeletal protein talin as well as those sequences which target the talin molecule to focal contacts. Using a series of overlapping talin-fusion proteins expressed in E. coli and 125I-vinculin in both gel-overlay and microtitre well binding assays, we present evidence for three separable binding sites for vinculin. All three are in the tail segment of talin (residues 434-2541) and are recognized by the same fragment of vinculin (residues 1-258). Two sites are adjacent to each other and span residues 498-950, and the third site is more than 700 residues distant in the primary sequence. Scatchard analysis of 125I-vinculin binding to talin also indicates three sites, each with a similar affinity (Kd = 2-6 x 10(-7) M). We also detect a substoichiometric interaction of higher affinity (Kd = 3 x 10(-8) M) which remains unexplained. By expressing regions of the chicken talin molecule in heterologous cells, we have shown that the sequences required to target talin to focal contacts overlap those which bind vinculin.
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