ArticleJournal of muscle research and cell motility1993
Effects of 2,3-butanedione monoxime on activation of contraction and crossbridge kinetics in intact and chemically skinned smooth muscle fibres from guinea pig taenia coli.
Article in Journal of muscle research and cell motility, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network.Cell motility and the cytoskeleton · 2009Article
- Origin of spontaneous activity in neonatal and adult rat bladders and its enhancement by stretch and muscarinic agonists.American journal of physiology. Renal physiology · 2007Article
- Effects of vanadate, phosphate and 2,3-butanedione monoxime (BDM) on skinned molluscan catch muscle.Pflugers Archiv : European journal of physiology · 2005Article
- Smooth, slow and smart muscle motors.Journal of muscle research and cell motility · 2003Review
- Inhibitory effect of 2,3-butanedione monoxime (BDM) on Na(+)/Ca(2+) exchange current in guinea-pig cardiac ventricular myocytes.British journal of pharmacology · 2001Article
- Mechanically induced avoidance response of chloroplasts in fern protonemal cells.Plant physiology · 1999Article
- Effects of 2,3-butanedione monoxime on excitation-contraction coupling in frog twitch fibres.Journal of muscle research and cell motility · 1998Article
- Temperature sensitivity of force and shortening velocity in maximally activated skinned smooth muscle.Journal of muscle research and cell motility · 1998Article
- Rho-stimulated contractility contributes to the fibroblastic phenotype of Ras-transformed epithelial cells.Molecular biology of the cell · 1997Article
- Rho-stimulated contractility drives the formation of stress fibers and focal adhesions.The Journal of cell biology · 1996Article
- The effects of caldesmon extraction on mechanical properties of skinned smooth muscle fibre preparations.Pflugers Archiv : European journal of physiology · 1996Article
- Myosin is involved in postmitotic cell spreading.The Journal of cell biology · 1995Article
- Effects of inorganic phosphate on cross-bridge kinetics at different activation levels in skinned guinea-pig smooth muscle.The Journal of physiology · 1995Article
- Comparison of the effects of 2,3-butanedione monoxime on force production, myosin light chain phosphorylation and chemical energy usage in intact and permeabilized smooth and skeletal muscles.Journal of muscle research and cell motility · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of 2,3-butanedione monoxime (BDM) were studied in smooth muscle fibres from guinea pig taenia coli. In intact muscle, active force during contractions induced by high-K+ was inhibited by about 10% in 1 mM BDM and by approximately 70% in 10 mM BDM. Intracellular [Ca2+] during contraction, measured with the fura-2 technique, was reduced in the presence of BDM. The reduction in force and [Ca2+] in the presence of 1 and 10 mM BDM could be reproduced by reduction in extracellular Ca2+, suggesting that BDM influences the Ca2+ entry or release. In skinned muscle preparations, BDM decreased the Ca2+ sensitivity of active force. This change could be explained by a decreased level of myosin light chain phosphorylation. In fibres maximally activated by thiophosphorylation, the effect of BDM on force occurred at higher concentrations; 10 mM gave no reduction of force and 60 mM 15% reduction. The maximal shortening velocity (Vmax) and force were unaffected by 30 mM BDM in thiophosphorylated muscle and decreased almost in parallel in Ca(2+)-activated contractions. The present results suggest that BDM inhibits myosin light chain phosphorylation, directly decreases force generation at the crossbridge level and inhibits the Ca2+ translocation in smooth muscle. The effect on force in skinned fibres is observed at higher BDM concentrations than those reported to be required for inhibition of force in striated muscle. The inhibition of force in intact smooth muscle could be explained by an influence on Ca2+ translocation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.