ArticleThe Journal of cell biology1993
Distinct functions of integrin alpha and beta subunit cytoplasmic domains in cell spreading and formation of focal adhesions.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- ITGB1 and DDR activation as novel mediators in acquired resistance to osimertinib and MEK inhibitors in EGFR-mutant NSCLC.Scientific reports · 2024Article
- Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions.Nature cell biology · 2023Article
- Curved adhesions mediate cell attachment to soft matrix fibres in 3D.bioRxiv : the preprint server for biology · 2023Article
- Enhancement of Farnesoid X Receptor Inhibits Migration, Adhesion and Angiogenesis through Proteasome Degradation and VEGF Reduction in Bladder Cancers.International journal of molecular sciences · 2022Article
- Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology.Molecular biology of the cell · 2020Review
- Integrin α11 cytoplasmic tail is required for FAK activation to initiate 3D cell invasion and ERK-mediated cell proliferation.Scientific reports · 2019Article
- The extreme C-terminal region of kindlin-2 is critical to its regulation of integrin activation.The Journal of biological chemistry · 2017Article
- The interaction of Gα13 with integrin β1 mediates cell migration by dynamic regulation of RhoA.Molecular biology of the cell · 2015Article
- The dual structural roles of the membrane distal region of the α-integrin cytoplasmic tail during integrin inside-out activation.Journal of cell science · 2015Article
- Integrin αII b tail distal of GFFKR participates in inside-out αII b β3 activation.Journal of thrombosis and haemostasis : JTH · 2014Article
- The mechanism of kindlin-mediated activation of integrin αIIbβ3.Current biology : CB · 2013Article
- Integrins regulate microtubule nucleating activity of centrosome through mitogen-activated protein kinase/extracellular signal-regulated kinase kinase/extracellular signal-regulated kinase (MEK/ERK) signaling.The Journal of biological chemistry · 2012Article
- Article
- Recruitment of vimentin to the cell surface by beta3 integrin and plectin mediates adhesion strength.Journal of cell science · 2009Article
- beta1 integrin cytoplasmic domain residues selectively modulate fibronectin matrix assembly and cell spreading through talin and Akt-1.The Journal of biological chemistry · 2009Article
- The cytoplasmic domain of anthrax toxin receptor 1 affects binding of the protective antigen.Infection and immunity · 2009Article
- Integrin cross-talk in endothelial cells is regulated by protein kinase A and protein phosphatase 1.The Journal of biological chemistry · 2008Article
- Integrins as regulators of the mitotic machinery.Current opinion in cell biology · 2008Review
- Key interactions in integrin ectodomain responsible for global conformational change detected by elastic network normal-mode analysis.Biophysical journal · 2008Article
- Requirement of alpha and beta subunit transmembrane helix separation for integrin outside-in signaling.Blood · 2007Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
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Abstract
Integrin-mediated cell adhesion often results in cell spreading and the formation of focal adhesions. We exploited the capacity of recombinant human alpha IIb beta 3 integrin to endow heterologous cells with the ability to adhere and spread on fibrinogen to study the role of integrin cytoplasmic domains in initiation of cell spreading and focal adhesions. The same constructs were also used to analyze the role of the cytoplasmic domains in maintenance of the fidelity of the integrin repertoire at focal adhesions. Truncation mutants of the cytoplasmic domain of alpha IIb did not interfere with the ability of alpha IIb beta 3 to initiate cell spreading and form focal adhesions. Nevertheless, deletion of the alpha IIb cytoplasmic domain allowed indiscriminate recruitment of alpha IIb beta 3 to focal adhesions formed by other integrins. Truncation of the beta 3 subunit cytoplasmic domain abolished cell spreading mediated by alpha IIb beta 3 and also abrogated recruitment of alpha IIb beta 3 to focal adhesions. This truncation also dramatically impaired the ability of alpha IIb beta 3 to mediate the contraction of fibrin gels. In contrast, the beta 3 subunit cytoplasmic truncation did not reduce the fibrinogen binding affinity of alpha IIb beta 3. Thus, the integrin beta 3 subunit cytoplasmic domain is necessary and sufficient for initiation of cell spreading and focal adhesion formation. Further, the beta 3 cytoplasmic domain is required for the transmission of intracellular contractile forces to fibrin gels. The alpha subunit cytoplasmic domain maintains the fidelity of recruitment of the integrins to focal adhesions and thus regulates their repertoire of integrins.
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