ArticleThe EMBO journal1994
Distinct structural elements of rab5 define its functional specificity.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 162 citations in OpenAlex.
- The small GTPase Rab5 inhibits actin polymerization mediated by the Legionella pneumophila effector VipA.Medical microbiology and immunology · 2026Article
- Structural basis for Rab6 activation by the Ric1-Rgp1 complex.Nature communications · 2024Article
- Structural basis for Rab6 activation by the Ric1-Rgp1 complex.bioRxiv : the preprint server for biology · 2024Article
- Polyisoprenylated cysteinyl amide inhibitors deplete singly polyisoprenylated monomeric G-proteins in lung and breast cancer cell lines.Oncotarget · 2023Article
- Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin.Small GTPases · 2020Article
- Structure and Dynamics of Mono- vs. Doubly Lipidated Rab5 in Membranes.International journal of molecular sciences · 2019Article
- Recognition and stabilization of geranylgeranylated human Rab5 by the GDP Dissociation Inhibitor (GDI).Small GTPases · 2019Article
- A Steric Gating Mechanism Dictates the Substrate Specificity of a Rab-GEF.Developmental cell · 2019Article
- Molecular control of Rab activity by GEFs, GAPs and GDI.Small GTPases · 2018Review
- Rab25 GTPase: Functional roles in cancer.Oncotarget · 2017Review
- Modifiers of prion protein biogenesis and recycling identified by a highly parallel endocytosis kinetics assay.The Journal of biological chemistry · 2017Article
- Numb regulates the balance between Notch recycling and late-endosome targeting in Drosophila neural progenitor cells.Molecular biology of the cell · 2016Article
- Endocytosis of Nanomedicines: The Case of Glycopeptide Engineered PLGA Nanoparticles.Pharmaceutics · 2015Review
- Phosphorylation of Rab5a protein by protein kinase Cϵ is crucial for T-cell migration.The Journal of biological chemistry · 2014Article
- Article
- Guanine nucleotide exchange factors (GEFs) have a critical but not exclusive role in organelle localization of Rab GTPases.The Journal of biological chemistry · 2013Article
- A Rab-centric perspective of bacterial pathogen-occupied vacuoles.Cell host & microbe · 2013Review
- Retrograde vesicle transport in the Golgi.Protoplasma · 2012Review
- Residues in the hendra virus fusion protein transmembrane domain are critical for endocytic recycling.Journal of virology · 2012Article
- The Ras protein superfamily: evolutionary tree and role of conserved amino acids.The Journal of cell biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the rab family of small GTPases are localized to distinct cellular compartments and function as specific regulators of vesicle transport between organelles. Overexpression of rab5, which is associated with early endosomes and the plasma membrane, increases the rate of endocytosis [Bucci et al. (1992) Cell, 70, 715-728]. From sequence alignments and molecular modelling we identified structural elements that might contribute to the definition of the functional specificity of rab5. To test the role of these elements experimentally, we transplanted them onto rab6, which is associated with the Golgi complex. The chimeric proteins were assayed for intracellular localization and stimulation of endocytosis. First, we found that the C-terminus of rab5 could target rab6 to the plasma membrane and early endosomes but it did not confer rab5-like stimulation of endocytosis. Further replacement of other regions revealed that the N-terminus, helix alpha 2/loop 5 and helix alpha 2/loop 7 were all required to functionally convert rab6 into rab5. Reciprocal hybrids of rab5 containing these regions replaced with those of rab6 were inactive, demonstrating that each region is essential for rab5 function. These results indicate that distinct structural elements specify the localization, membrane association and regulatory function of rab5.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.