ArticleThe American journal of pathology1994
Induction of alpha-smooth muscle actin expression in cultured human brain pericytes by transforming growth factor-beta 1.
Article in The American journal of pathology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 189 citations in OpenAlex.
- The effect of an adenosine AFluids and barriers of the CNS · 2018Trial
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- Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding.Science translational medicine · 2024Article
- Dual gene-activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin-1.Bioengineering & translational medicine · 2023Article
- Endothelial Cell-Pericyte Interactions in the Pathogenesis of Cerebral Cavernous Malformations (CCMs).Cold Spring Harbor perspectives in medicine · 2023Review
- Adventitia-derived extracellular matrix hydrogel enhances contractility of human vasa vasorum-derived pericytes via αJournal of biomedical materials research. Part A · 2022Article
- Validation of Specific and Reliable Genetic Tools to Identify, Label, and Target Cardiac Pericytes in Mice.Journal of the American Heart Association · 2022Article
- Review
- Neural crest cell-derived pericytes act as pro-angiogenic cells in human neocortex development and gliomas.Fluids and barriers of the CNS · 2021Review
- A protocol for rapid pericyte differentiation of human induced pluripotent stem cells.STAR protocols · 2021Article
- Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model.Nature communications · 2021Article
- The Ferroxidase Hephaestin in Lung Cancer: Pathological Significance and Prognostic Value.Frontiers in oncology · 2021Article
- Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.FEBS open bio · 2021Article
- Impact of Platelet Count in Retinopathy of Prematurity.Turkish journal of ophthalmology · 2020Article
- A subtype of cerebrovascular pericytes is associated with blood-brain barrier disruption that develops during normal aging and simian immunodeficiency virus infection.Neurobiology of aging · 2020Article
- Generation of a novel mouse strain with fibroblast-specific expression of Cre recombinase.Matrix biology plus · 2020Article
- Advancing brain barriers RNA sequencing: guidelines from experimental design to publication.Fluids and barriers of the CNS · 2020Review
- Reduced Influence of apoE on Aβ43 Aggregation and Reduced Vascular Aβ43 Toxicity as Compared with Aβ40 and Aβ42.Molecular neurobiology · 2020Article
- Cerebral amyloid angiopathy and Alzheimer disease - one peptide, two pathways.Nature reviews. Neurology · 2020Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pericytes are cells localized at the abluminal side of the microvascular endothelium and completely enveloped by a basement membrane. Pericytes have close contact with endothelial cells and are probably involved in the regulation of endothelial cell functions. Previous studies suggested a role for pericytes in microvascular proliferation in tumors. To study this cell type, we isolated human brain pericytes from microvessel segments derived from autopsy brain tissue. These cells were characterized in vitro using a panel of monoclonal antibodies. Human brain pericytes were reactive with monoclonal antibodies directed against the high molecular weight-melanoma associated antigen and intercellular adhesion molecule-1, but only a minority of the cells expressed alpha-smooth muscle actin (alpha-SMA, 0 to 10%) or vascular cell adhesion molecule-1 (10 to 50%). In histologically normal human brain microvessels in situ, pericytes consistently lacked staining for these four markers. Tissue with microvascular proliferation, however, showed a marked pericyte staining for both alpha-SMA and high molecular weight-melanoma associated antigen. The expression of alpha-SMA in vitro could be slightly up-regulated by incubation with serum-containing medium. An increase in alpha-SMA expression up to 40% of the total cell population was seen when pericytes were treated with transforming growth factor-beta 1, whereas basic fibroblast growth factor slightly inhibited alpha-SMA expression. Incubation with other factors (platelet-derived growth factor-AA, heparin, interferon-gamma, tumor necrosis factor-alpha) had no effect on the alpha-SMA expression at all. Transforming growth factor-beta 1 thus induces smooth muscle-like differentiation in pericytes in vitro and might play a role in the activation of pericytes during angiogenesis in vivo.
Indexed as
Identifiers
8311120PMC1887139W1577346867What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.