ArticlePflugers Archiv : European journal of physiology1993
Effect of secretin and inhibitors of HCO3-/H+ transport on the membrane voltage of rat pancreatic duct cells.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular mechanism of pancreatic and salivary gland fluid and HCO3 secretion.Physiological reviews · 2012Review
- Phosphorylation-induced modulation of pNBC1 function: distinct roles for the amino- and carboxy-termini.The Journal of physiology · 2003Article
- Membrane potential and bicarbonate secretion in isolated interlobular ducts from guinea-pig pancreas.The Journal of general physiology · 2002Article
- The stoichiometry of the electrogenic sodium bicarbonate cotransporter pNBC1 in mouse pancreatic duct cells is 2 HCO(3)(-):1 Na(+).The Journal of physiology · 2001Article
- CO2 permeability and bicarbonate transport in microperfused interlobular ducts isolated from guinea-pig pancreas.The Journal of physiology · 2000Article
- Accumulation of intracellular HCO3- by Na(+)-HCO3- cotransport in interlobular ducts from guinea-pig pancreas.The Journal of physiology · 1996Article
- Bicarbonate secretion in interlobular ducts from guinea-pig pancreas.The Journal of physiology · 1996Article
- Characterization of single potassium channels in mouse pancreatic acinar cells.The Journal of physiology · 1995Article
- Localization of carbonic anhydrase in the salivary glands of the cockroach, Periplaneta americana.Histochemistry · 1994Article
- pH dependence of K+ conductances of rat cortical collecting duct principal cells.Pflugers Archiv : European journal of physiology · 1994Article
- pH regulation in HT29 colon carcinoma cells.Pflugers Archiv : European journal of physiology · 1994Article
- Effect of ATP, carbachol and other agonists on intracellular calcium activity and membrane voltage of pancreatic ducts.Pflugers Archiv : European journal of physiology · 1994Article
- Effect of vasoactive intestinal peptide, carbachol and other agonists on the membrane voltage of pancreatic duct cells.Pflugers Archiv : European journal of physiology · 1993Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to study the effect of secretin on the electrophysiological response of pancreatic ducts. Furthermore, we investigated the effects of lipid-soluble buffers and inhibitors of HCO3-/H+ transport. Ducts obtained from fresh rat pancreas were perfused in vitro. Secretin depolarized the basolateral membrane voltage, Vbl, by up to 35 mV (n = 37); a half-maximal response was obtained at 3 x 10(-11) mol/l. In unstimulated ducts a decrease in the luminal Cl- concentration (120 to 37 mmol/l) had a marginal effect on Vbl, but after maximal secretin stimulation it evoked a 14 +/- 2 mV depolarization (n = 6), showing that a luminal Cl- conductance (GCl-) was activated. The depolarizing effect of secretin on Vbl was often preceded by about a 6 mV hyperpolarization, most likely due to an increase in the basolateral GK+. Perfusion of ducts with DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid, 0.01 mmol/l) or addition of ethoxzolamide (0.1 mmol/l) to the bath medium diminished the effect of secretin. Acetate or pre-treatment of ducts with NH4+/NH3 (10 mmol/l in the bath) depolarized the resting Vbl of -65 +/- 2 mV by 16 +/- 4 mV (n = 7) and 19 +/- 3 mV (n = 10), respectively. The fractional resistance of the basolateral membrane (FRbl) doubled, and the depolarizing responses to changes in bath K+ concentrations (5 to 20 mmol/l) decreased from 22 +/- 1 to 11 +/- 2 mV.(ABSTRACT TRUNCATED AT 250 WORDS)
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