ArticlePflugers Archiv : European journal of physiology1993
Basolateral mechanisms of intracellular pH regulation in the colonic epithelial cell line HT29 clone 19A.
Article in Pflugers Archiv : European journal of physiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 20 citations in OpenAlex.
- Platelet-activating factor-induced chloride channel activation is associated with intracellular acidosis and apoptosis of intestinal epithelial cells.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- The effects of dietary phosphorus deficiency on surface pH and membrane composition of the mucosa epithelium in caprine jejunum.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2007Article
- Carbon dioxide differentially affects the cytokine release of macrophage subpopulations exclusively via alteration of extracellular pH.Surgical endoscopy · 2006Article
- Bicarbonate exporting transporters in the ovine ruminal epithelium.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2005Article
- Interferon-gamma increases hPepT1-mediated uptake of di-tripeptides including the bacterial tripeptide fMLP in polarized intestinal epithelia.The American journal of pathology · 2003Article
- Maintenance and regulation of the pH microclimate at the luminal surface of the distal colon of guinea-pig.The Journal of physiology · 1999Article
- Effect of alkalinization of cytosolic pH by amines on intracellular Ca2+ activity in HT29 cells.Pflugers Archiv : European journal of physiology · 1996Article
- pH regulation in HT29 colon carcinoma cells.Pflugers Archiv : European journal of physiology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular pH (pHi) of the colonic tumour cell line HT29 cl.19A was studied by microspectrofluorometry using the pH-sensitive dye BCECF. Single cells within a confluent monolayer, grown in a polarized manner on permeable supports, were examined. An amiloride-sensitive Na+/H+ exchange and a stilbene-insensitive Cl-/HCO3- exchange mechanism have been identified in the basolateral membrane. Removal of Na+ from the basolateral solution caused a decrease of pHi by 0.50 +/- 0.09 unit (n = 4). Amiloride or Na(+)-free solution at the apical side had no effect on pHi. Cl- removal at the basolateral side led to an increase of pHi by 0.20 +/- 0.03 unit (n = 4) whereas apical removal had no influence on pHi. This effect was independent of Na+ and was insensitive to 0.2 mM 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulphonic acid. A basolateral Cl-/HCO3- exchanger is the most likely explanation for this observation. The Na+/H+ exchange mechanism in the basolateral membrane is an acid extruder, whereas the Cl-/HCO3- exchanger is an acid loader. Both of these mechanisms are important for the maintenance of intracellular pH in HT29 cl.19A cells.
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