ArticleHistochemistry1993
Immuno- and lectin histochemistry of epithelial subtypes and their changes in a radiation-induced lung fibrosis model of the mini pig.
Article in Histochemistry, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Potential contribution of alveolar epithelial type I cells to pulmonary fibrosis.Bioscience reports · 2017Review
- Article
- Dual-immunohistochemistry provides little evidence for epithelial-mesenchymal transition in pulmonary fibrosis.Histochemistry and cell biology · 2008Article
- Epithelial vs myofibroblast differentiation in immortal rat lung cell lines--modulating effects of bleomycin.Histochemistry and cell biology · 2005Article
- A new rat type I-like alveolar epithelial cell line R3/1: bleomycin effects on caveolin expression.Histochemistry and cell biology · 2004Article
- Localization of the peptide transporter PEPT2 in the lung: implications for pulmonary oligopeptide uptake.The American journal of pathology · 2001Article
- Monolayers of human alveolar epithelial cells in primary culture for pulmonary absorption and transport studies.Pharmaceutical research · 1999Article
- Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs.Histochemistry and cell biology · 1996Article
- Immunohistochemical evidence for loss of ICAM-1 by alveolar epithelial cells in pulmonary fibrosis.Histochemistry and cell biology · 1995Article
- Distribution of E-cadherin and Ep-CAM in the human lung during development and after injury.Histochemistry and cell biology · 1995Article
- Immunohistological detection of the beta subunit of prolyl 4-hydroxylase in rat and mini pig lungs with radiation-induced pulmonary fibrosis.Virchows Archiv : an international journal of pathology · 1994Article
- Colocalization of cytokeratin 18 and villin in type III alveolar cells (brush cells) of the rat lung.Histochemistry · 1994Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell types of lung epithelia of mini pigs have been studied using a panel of monoclonal and polyclonal antibodies against cytokeratins (CKs) and vimentin and three lectins before and after radiation-induced fibrosis. In normal tissues, CK18 specific antibodies reacted above all with type II alveolar epithelial cells, while CK7 and pan CK-specific antibodies stained the whole alveolar epithelium. In bronchial epithelial cells, CKs 7, 8, 18 and focally CKs 4 and 13 as well as vimentin were found. Cell specificity of the CK pattern was confirmed by double label immunofluorescence using type II cell-specific Maclura pomifera (MPA) lectin, type I cell specific Lycopersicon esculentum (LEA) lectin and capillary endothelium-binding Dolichos biflorus (DBA) lectin. In experimental pulmonary fibrosis, enhanced coexpression of CK and vimentin was observed in bronchial epithelium. Subtypes of alveolar epithelial cells were no longer easily distinguishable. CK18 was found to be expressed in the entire alveolar epithelium. The gradual loss of the normal alveolar epithelial marker, as seen by the binding of MPA to type I-like cells, of LEA to type II-like cells and the partial loss of MPA-binding to type II cells, was paralleled by the appearance of CK4, typical for squamous epithelia, and the occurrence of DBA-binding in epithelial cells. Implications of these results for general concepts of intermediate filament protein expression and lectin binding in the fibrotic process are discussed.
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