ArticleThe EMBO journal1994
Nuclear restoration of cytoplasmic male sterility in sunflower is associated with the tissue-specific regulation of a novel mitochondrial gene.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 189 citations in OpenAlex.
- Comparison of Mitochondrial Genomes between a Cytoplasmic Male-Sterile Line and Its Restorer Line for Identifying Candidate CMS Genes inInternational journal of molecular sciences · 2022Article
- Comparative Transcriptome Analysis between a Novel Allohexaploid Cotton Progeny CMS Line LD6A and Its Maintainer Line LD6B.International journal of molecular sciences · 2019Article
- Organelle genome composition and candidate gene identification for Nsa cytoplasmic male sterility in Brassica napus.BMC genomics · 2019Article
- Development and Validation of Markers for the Fertility Restorer GeneInternational journal of molecular sciences · 2019Article
- Characterization of the mitochondrial genome of the MAX1 type of cytoplasmic male-sterile sunflower.BMC plant biology · 2019Article
- Molecular and Functional Diversity of RNA Editing in Plant Mitochondria.Molecular biotechnology · 2018Review
- Recombination Events Involving the atp9 Gene Are Associated with Male Sterility of CMS PET2 in Sunflower.International journal of molecular sciences · 2018Article
- Article
- Sunflower Hybrid Breeding: From Markers to Genomic Selection.Frontiers in plant science · 2017Review
- Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues.Plant methods · 2017Article
- Article
- Maize Male sterile 8 (Ms8), a putative β-1,3-galactosyltransferase, modulates cell division, expansion, and differentiation during early maize anther development.Plant reproduction · 2013Article
- Article
- An unedited 1.1 kb mitochondrial orfB gene transcript in the wild abortive cytoplasmic male sterility (WA-CMS) system of Oryza sativa L. subsp. indica.BMC plant biology · 2010Article
- Article
- Mitochondrial nad2 gene is co-transcripted with CMS-associated orfB gene in cytoplasmic male-sterile stem mustard (Brassica juncea).Molecular biology reports · 2009Article
- Genome barriers between nuclei and mitochondria exemplified by cytoplasmic male sterility.Plant & cell physiology · 2008Review
- Introgression and molecular tagging of Rf (4), a new male fertility restoration gene from wild sunflower Helianthus maximiliani L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.).Plant molecular biology · 2007Article
- Transcript levels in plant mitochondria show a tight homeostasis during day and night.Molecular genetics and genomics : MGG · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that cytoplasmic male sterility in sunflower is associated with the insertion into the mitochondrial DNA of a novel open reading frame (ORF) located 3' to the atpA gene. Here, we show that in mitochondria from the sterile line, this novel ORF (ORF522) is cotranscribed with atpA. We have identified the product of the ORF522 as being a 15 kDa protein previously observed in sterile plant mitochondria by in organello translation. Both Western blot analysis and in organello translation assays show reduced levels of the 15 kDa polypeptide upon restoration of fertility. Interestingly, this reduction is tissue specific since it is only observed in the male florets from restored hybrid plants. These results suggest that the 15 kDa novel polypeptide is probably responsible for the CMS phenotype. Northern blot analysis using RNA from both seedlings and male florets shows a flower-specific reduction in the level of the ORF522 transcript in the restored hybrid line. The reduction is not due to a reduced transcription rate as demonstrated by 'run-on' experiments using mitochondria isolated from male florets. This suggests that the product of the nuclear restorer gene acts at the post-transcriptional level to destabilize the novel mitochondrial transcript in a tissue-specific manner and restore male fertility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.