ArticleThe EMBO journal1994
In vitro nuclear import of snRNPs: cytosolic factors mediate m3G-cap dependence of U1 and U2 snRNP transport.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 69 citations in OpenAlex.
- TGS1 impacts snRNA 3'-end processing, ameliorates survival motor neuron-dependent neurological phenotypes in vivo and prevents neurodegeneration.Nucleic acids research · 2022Article
- Article
- An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier.The Journal of cell biology · 2013Article
- Identification and characterization of Drosophila Snurportin reveals a role for the import receptor Moleskin/importin-7 in snRNP biogenesis.Molecular biology of the cell · 2013Article
- Cellular cofactors of lentiviral integrase: from target validation to drug discovery.Molecular biology international · 2012Article
- Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells.Molecular biology of the cell · 2006Article
- Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies.Molecular biology of the cell · 2006Article
- Cross-talk between snurportin1 subdomains.Molecular biology of the cell · 2005Article
- Importin beta-depending nuclear import pathways: role of the adapter proteins in the docking and releasing steps.Molecular biology of the cell · 2003Article
- SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta.Human molecular genetics · 2002Article
- The importin-beta binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro.The Journal of cell biology · 2002Article
- High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- RNA-mediated interaction of Cajal bodies and U2 snRNA genes.The Journal of cell biology · 2001Article
- Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins.The Plant cell · 2001Article
- The box C/D motif directs snoRNA 5'-cap hypermethylation.Nucleic acids research · 2000Article
- The spinal muscular atrophy disease gene product, SMN: A link between snRNP biogenesis and the Cajal (coiled) body.The Journal of cell biology · 1999Article
- Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences.The Journal of biological chemistry · 1999Article
- A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing.Molecular and cellular biology · 1999Article
- Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.Molecular biology of the cell · 1998Article
- Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure.The EMBO journal · 1998Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established an in vitro snRNP nuclear import system using digitonin permeabilized somatic cells supplemented with cytosolic extracts. As model karyophiles we used digoxygenin labelled U1 snRNPs or fluorescein labelled U2 snRNPs. In vitro nuclear import of snRNPs is inhibited by anti-pore component antibodies, consistent with transport occurring through nuclear pores. This import requires ATP, cytosolic factors and a nuclear localization signal (NLS). SnRNP nuclear accumulation is saturable and distinct from protein transport. Nuclear import of snRNPs, in permeabilized NRK cells supplemented with somatic cell cytosol, requires the same NLS structures as those identified in micro-injected mammalian cells. In contrast to the situation in Xenopus oocytes, the m3G-cap is not required for in vitro nuclear import of U1 and U2 snRNPs in somatic cells. Instead, assembly of the Sm-core domain is both necessary and sufficient to mediate snRNP nuclear targeting. Interestingly, when the in vitro system was provided with cytosol from Xenopus oocytes instead of somatic cells, U1 and U2 snRNP nuclear import was provided with cytosol from Xenopus oocytes instead of somatic cells, U1 and U2 snRNP nuclear import was m3G-cap dependent. These results indicate that soluble cytosolic factors mediate the differential m3G-cap dependence of U1 and U2 snRNP nuclear import in somatic cells and oocytes. We also demonstrate the existence of a soluble cytosolic factor whose interaction with the U2 snRNP m3G-cap is both saturable and essential for U2 snRNP nuclear import in Xenopus oocytes.
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