ArticleChromosoma1993
Atomic force microscopy of mammalian sperm chromatin.
Article in Chromosoma, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed.
- Spatiotemporal dynamics of protamine-DNA condensation revealed by high-speed atomic force microscopy.Nucleic acids research · 2025Article
- Biophysical ordering transitions underlie genome 3D re-organization during cricket spermiogenesis.Nature communications · 2023Article
- Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements.Frontiers in cell and developmental biology · 2021Article
- Role of Disulfide Bonds on DNA Packaging Forces in Bull Sperm Chromatin.Biophysical journal · 2017Article
- Proteomics and the genetics of sperm chromatin condensation.Asian journal of andrology · 2011Review
- Sperm chromatin: fertile grounds for proteomic discovery of clinical tools.Molecular & cellular proteomics : MCP · 2008Review
- Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology.Pflugers Archiv : European journal of physiology · 2008Review
- Bacterial protein HU dictates the morphology of DNA condensates produced by crowding agents and polyamines.Nucleic acids research · 2007Article
- Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa.Journal of nanobiotechnology · 2005Article
- Chromosomal proteins in the spermatogenesis of Drosophila.Chromosoma · 2003Review
- Combined nanomanipulation by atomic force microscopy and UV-laser ablation for chromosomal dissection.European biophysics journal : EBJ · 2003Article
- Combining constitutive materials modeling with atomic force microscopy to understand the mechanical properties of living cells.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- AFM analysis of DNA-protamine complexes bound to mica.Nucleic acids research · 1997Article
- Well-defined genome architecture in the human sperm nucleus.Chromosoma · 1995Article
- Probing specific molecular conformations with the scanning force microscope. Complexes of plasmid DNA and anti-Z-DNA antibodies.Nucleic acids research · 1994Article
- Probing chromatin with the scanning force microscope.Chromosoma · 1994Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used the atomic force microscope (AFM) to image the surfaces of intact bull, mouse and rat sperm chromatin and partially decondensed mouse sperm chromatin attached to coverglass. High resolution AFM imaging was performed in air and saline using uncoated, unfixed and unstained chromatin. Images of the surfaces of intact chromatin from all three species and of an AFM-dissected bull sperm nucleus have revealed that the DNA is organized into large nodular subunits, which vary in diameter between 50 and 100 nm. Other images of partially decondensed mouse sperm chromatin show that the nodules are arranged along thick fibers that loop out away from the nucleus upon decondensation. These fibers appear to stretch or unravel, generating narrow smooth fibers with thicknesses equivalent to a single DNA-protamine complex. High resolution AFM images of the nodular subunits suggest that they are discrete, ellipsoid-shaped DNA packaging units possibly only one level of packaging above the protamine-DNA complex.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.