ArticleClinical and experimental immunology1994
Activated neutrophils express proteinase 3 on their plasma membrane in vitro and in vivo.
Article in Clinical and experimental immunology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers, 1 of them a synthesis that pooled it.
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Who cites it
86 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Associations of HLA genotypes with antithyroid drug-induced agranulocytosis: A systematic review and meta-analysis of pharmacogenomics studies.British journal of clinical pharmacology · 2019Pooled it
- Structures of proteinase 3 and the CD177 receptor complex reveal a major autoantibody epitope.EMBO reports · 2026Article
- Choline supplementation protects against sepsis-induced lung injury, potentially through suppression of Prtn3-associated monocyte activation.Frontiers in pharmacology · 2026Article
- Avacopan is effective in inducing remission for MPA/GPA, regardless of changes in serum C5a levels: a single-center study in Japan.BMC rheumatology · 2025Article
- CCL23 is a potential biomarker for antineutrophil cytoplasmic antibody-associated vasculitis.Arthritis research & therapy · 2025Article
- The Genetic Variation of Porcine Reproductive and Respiratory Syndrome Virus Replicase Protein nsp2 Modulates Viral Virulence and Persistence.Journal of virology · 2023Article
- Mechanisms of vascular damage in ANCA vasculitis.Seminars in immunopathology · 2022Review
- Leveling Up the Controversial Role of Neutrophils in Cancer: When the Complexity Becomes Entangled.Cells · 2021Review
- Fulminant granulomatosis with polyangiitis presenting with diffuse alveolar haemorrhage following COVID-19.BMJ case reports · 2021Article
- Granulomatous Inflammation in ANCA-Associated Vasculitis.International journal of molecular sciences · 2021Review
- Association of MICA gene polymorphisms with thionamide-induced agranulocytosis.Journal of endocrinological investigation · 2021Article
- Autoantibodies Targeting Intracellular and Extracellular Proteins in Autoimmunity.Frontiers in immunology · 2021Review
- Reduced neutrophil granular proteins and post-treatment modulation in tuberculous lymphadenitis.PloS one · 2021Article
- Immunopathogenesis of ANCA-Associated Vasculitis.International journal of molecular sciences · 2020Review
- Neutrophil Extracellular Traps (NETs) Take the Central Stage in Driving Autoimmune Responses.Cells · 2020Review
- Autoantibody Biomarkers in Rheumatic Diseases.International journal of molecular sciences · 2020Review
- Therapeutic Myeloperoxidase Inhibition Attenuates Neutrophil Activation, ANCA-Mediated Endothelial Damage, and Crescentic GN.Journal of the American Society of Nephrology : JASN · 2020Article
- Role of Neutrophils in Systemic Vasculitides.Frontiers in immunology · 2020Review
- Understanding the role of neutrophils in chronic inflammatory airway disease.F1000Research · 2019Review
- Proteinase 3; a potential target in chronic obstructive pulmonary disease and other chronic inflammatory diseases.Respiratory research · 2018Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Apart from the diagnostic value of anti-neutrophil cytoplasmic antibodies (ANCA), their detailed characterization and that of their corresponding antigens have opened new ways for the exploration of the pathogenesis of primary systemic vasculitis. ANCA are now thought to play an important functional role via activation of phagocytic cells (e.g. polymorphonuclear neutrophils (PMN)). In this study we examined the mechanisms by which ANCA could gain access to proteinase 3 (PR3) in intact PMN, at two levels: ex vivo by analysing the presence of PR3 on the plasma membrane of PMN from patients with ANCA-associated vasculitis, and in vitro by stimulation of PMN using different cytokines, including recombinant tumour necrosis factor-alpha (rhTNF-alpha) and two forms of IL-8 (produced by monocytic and endothelial cells). Using immunocytochemical staining techniques (FACS and immunoelectronmicroscopy) PR3 has been detected on the plasma membrane of PMN from patients with active ANCA-associated vasculitis. However, this phenomenon is also seen in patients with sepsis who do not have ANCA. In addition, TNF-alpha and both forms of IL-8 act synergistically and induce a translocation of PR3 from the intragranular loci to the cell surface of PMN. These results provide strong evidence for the hypothesis that ANCA are directly pathogenic by binding to PR3 which is expressed on the cell surface of primed/activated PMN.
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