ArticleBritish journal of pharmacology1993
Increases in intracellular calcium via activation of an endogenous P2-purinoceptor in cultured CHO-K1 cells.
Article in British journal of pharmacology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Investigation of reported small molecule P2Y6 receptor antagonists.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2026Article
- Characterization and deorphanization of RYamide signaling in Aedes aegypti: A potential regulator of hindgut-associated physiology.PloS one · 2026Article
- Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis ineLife · 2025Article
- Dynamics of release and activity of select neuropeptides post-bloodmeal in the female mosquito, Aedes aegypti.The Journal of experimental biology · 2025Article
- Sequence, characterization and pharmacological analyses of the adipokinetic hormone receptor in the stick insect,Frontiers in endocrinology · 2025Article
- A nondestructive membrane engineering method using an amphiphilic polymer.Protein science : a publication of the Protein Society · 2024Article
- A Set of Experimentally Validated Decoys for the Human CC Chemokine Receptor 7 (CCR7) Obtained by Virtual Screening.Frontiers in pharmacology · 2022Article
- A novel bioassay to detect Nociceptin/Orphanin FQ release from single human polymorphonuclear cells.PloS one · 2022Article
- Receptor Characterization and Functional Activity of Pyrokinins on the Hindgut in the Adult Mosquito,Frontiers in physiology · 2020Article
- Molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, Aedes aegypti.Scientific reports · 2018Article
- Differential effects of the Gβ5-RGS7 complex on muscarinic M3 receptor-induced Ca2+ influx and release.Molecular pharmacology · 2014Article
- Purinergic signalling in the reproductive system in health and disease.Purinergic signalling · 2014Review
- Impact of polymorphic variants on the molecular pharmacology of the two-agonist conformations of the human β1-adrenoceptor.PloS one · 2013Article
- Comparative analysis of calcium spikes upon activation of serotonin(1A) and purinergic receptors.PloS one · 2012Article
- Purinergic signalling mobilizes mitochondrial Ca²⁺ in mouse Sertoli cells.The Journal of physiology · 2011Article
- Divergent effect of mammalian PLCζ in generating Ca²⁺ oscillations in somatic cells compared with eggs.The Biochemical journal · 2011Article
- Heat shock enhances CMV-IE promoter-driven metabotropic glutamate receptor expression and toxicity in transfected cells.Neuropharmacology · 2011Article
- Acid stimulation (sour taste) elicits GABA and serotonin release from mouse taste cells.PloS one · 2011Article
- Amphioxus expresses both vertebrate-type and invertebrate-type dopamine D(1) receptors.Invertebrate neuroscience : IN · 2010Article
- Mitochondrial reactive oxygen species mediate GPCR-induced TACE/ADAM17-dependent transforming growth factor-alpha shedding.Molecular biology of the cell · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Increases in intracellular calcium ([Ca2+]i) were measured in chinese hamster cultured ovary cells (clone, CHO-K1), by use of the fluorescent, calcium-sensitive dye, fura-2. 2. Addition of both ATP and UTP elicited rapid increases in [Ca2+]i due to mobilization from intracellular stores and calcium entry across the plasma membrane. 3. Omission of calcium from the extracellular medium and pre-incubation with the inorganic calcium channel blocker, nickel (Ni2+) prevented the calcium entry components of the responses. 4. Investigation of the concentration-response relationships of various analogues of ATP suggests the presence of a purinoceptor which cannot be characterized as P2X or P2Y. In addition, there appears to be a sub-population of P2Y-purinoceptors which do not cross-react with the 'nucleotide' receptor population. 5. Cross-desensitization and additivity experiments suggest that both ATP and UTP activate the same receptor. 6. Pre-incubation with the tumour-promoting agent, beta-phorbol-12,13 dibutyrate (PDBu), caused a reduction in the increases in [Ca2+]i, suggesting a role for protein kinase C in feedback inhibition of purinoceptor responses in this cell line. 7. In summary, we present evidence for the existence of an endogenous P2U-purinoceptor (or 'nucleotide receptor') which is linked to increases in [Ca2+]i in CHO-K1 cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.