ArticleMolecular biology of the cell1993
Regulation of NADPH oxidase activity by Rac GTPase activating protein(s).
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 92 citations in OpenAlex.
- RAC2 orchestrates immune cell actin remodeling and migration.Frontiers in immunology · 2026Review
- The small GTPase Rap1A expedites the NOX2 oxidative burst by facilitating Rac and NOX2 autoactivations.The FEBS journal · 2025Article
- NADPH Oxidase 3: Beyond the Inner Ear.Antioxidants (Basel, Switzerland) · 2024Review
- Article
- The role of ROS in tumour development and progression.Nature reviews. Cancer · 2022Review
- CD47 and Nox1 Mediate Dynamic Fluid-Phase Macropinocytosis of Native LDL.Antioxidants & redox signaling · 2017Article
- Statins in anthracycline-induced cardiotoxicity: Rac and Rho, and the heartbreakers.Cell death & disease · 2017Review
- NADPH Oxidase Deficiency: A Multisystem Approach.Oxidative medicine and cellular longevity · 2017Review
- Activation of AKT pathway by Nrf2/PDGFA feedback loop contributes to HCC progression.Oncotarget · 2016Article
- Regulation of NADPH oxidases in skeletal muscle.Free radical biology & medicine · 2016Review
- Systematic analysis of copy number variants of a large cohort of orofacial cleft patients identifies candidate genes for orofacial clefts.Human genetics · 2016Article
- NADPH oxidases: an overview from structure to innate immunity-associated pathologies.Cellular & molecular immunology · 2015Review
- Role of the Rho GTPase Rac in the activation of the phagocyte NADPH oxidase: outsourcing a key task.Small GTPases · 2014Review
- Neuregulin mediates F-actin-driven cell migration through inhibition of protein kinase D1 via Rac1 protein.The Journal of biological chemistry · 2013Article
- The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities.Antioxidants & redox signaling · 2012Review
- Ras-induced senescence and its physiological relevance in cancer.Current cancer drug targets · 2010Review
- Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Reactive oxygen species: a double-edged sword in oncogenesis.World journal of gastroenterology · 2009Review
- MKK6 phosphorylation regulates production of superoxide by enhancing Rac GTPase activity.Antioxidants & redox signaling · 2007Article
- Tobacco nectaries express a novel NADPH oxidase implicated in the defense of floral reproductive tissues against microorganisms.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Activation of the NADPH oxidase of phagocytic cells requires the action of Rac2 or Rac1, members of the Ras superfamily of GTP-binding proteins. Rac proteins are active when in the GTP-bound form and can be regulated by a variety of proteins that modulate the exchange of GDP for GTP and/or GTP hydrolysis. The p190 Rac GTPase Activating Protein (GAP) inhibits human neutrophil NADPH oxidase activity in a cell-free assay system with a K1 of approximately 100 nM. Inhibition by p190 was prevented by GTP gamma S, a nonhydrolyzable analogue of GTP. Similar inhibition was seen with a second protein exhibiting Rac GAP activity, CDC42Hs GAP. The effect of p190 on superoxide (O2-) formation was reversed by the addition of a constitutively GTP-bound Rac2 mutant or Rac1-GTP gamma S but not by RhoA-GTP gamma S. Addition of p190 to an activated oxidase produced no inhibitory effect, suggesting either that p190 no longer has access to Rac in the assembled oxidase or that Rac-GTP is not required for activity once O2- generation has been initiated. These data confirm the role of Rac in NADPH oxidase regulation and support the view that it is the GTP form of Rac that is necessary for oxidase activation. Finally, they raise the possibility that NADPH oxidase may be regulated by the action of GAPs for Rac proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.