ArticleJournal of anatomy1993
Novel peptides from adrenomedullary chromaffin vesicles.
Article in Journal of anatomy, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- ProSAAS neuropeptides and receptors GPR171 and GPR83: Potential therapeutic applications for pain, anxiety, and body weight regulation.The Journal of pharmacology and experimental therapeutics · 2025Review
- Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Mass spectrometry-based neuropeptidomics of secretory vesicles from human adrenal medullary pheochromocytoma reveals novel peptide products of prohormone processing.Journal of proteome research · 2010Article
- Homology modeling and site-directed mutagenesis to identify selective inhibitors of endothelin-converting enzyme-2.Journal of medicinal chemistry · 2008Article
- Animals lacking endothelin-converting enzyme-2 are deficient in learning and memory.Genes, brain, and behavior · 2008Article
- Neuropeptide-processing enzymes: applications for drug discovery.The AAPS journal · 2005Review
- Characterization of endothelin-converting enzyme-2. Implication for a role in the nonclassical processing of regulatory peptides.The Journal of biological chemistry · 2003Article
- Processing of proSAAS in neuroendocrine cell lines.The Biochemical journal · 2002Article
- Identification of peptides from brain and pituitary of Cpe(fat)/Cpe(fat) mice.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Identification and characterization of proSAAS, a granin-like neuroendocrine peptide precursor that inhibits prohormone processing.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Kinetics of neuroendocrine differentiation in an androgen-dependent human prostate xenograft model.The American journal of pathology · 1999Article
- Novel autocrine feedback control of catecholamine release. A discrete chromogranin a fragment is a noncompetitive nicotinic cholinergic antagonist.The Journal of clinical investigation · 1997Article
- Accumulation of Hg(II) Ions in Mouse Adrenal Gland.Pathology oncology research : POR · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adrenal medulla chromaffin vesicle (CV) contains, on a weight basis, as much soluble protein and peptide as catecholamine. The bulk of the protein is accounted for by chromogranins (Cgr) A, B and C. Additionally, a large variety of neuropeptides and their precursor proteins have been found recently within these vesicles. Nevertheless, fractionation of CV lysates indicates the presence of many more peptides than previously reported. In the hope of finding novel bioactive peptides, we initiated a systematic isolation and characterisation of CV peptides. Bovine CV pellets were prepared by sucrose gradient centrifugation and immediately boiled in water to avoid degradation of native proteins and peptides. The water lysates were fractionated through a battery of reversed-phase and ion-exchange high-performance chromatographic steps. We fully or partially characterised a substantial number of novel peptides derived from CgrA and CgrB. A tetradecapeptide and a 13 kDa extended peptide were derived from the bovine homologue of rat secretogranin III. Peptides corresponding to C-terminal fragments of 7B2 and proteoglycan II were also found. Additionally, several sequences had no known precursors. Of the sequences derived from known precursors some corresponded to fragments bracketed by pairs of basic amino acids, but others were preceded or followed by single basic residues or by unusual putative cleavage sites. Some of these peptides were postranslationally modified (pyroglutamylation, glycosylation, phosphorylation, amidation). A significant degree of structural conservation of some of these peptides across species suggests that they may exert biological effects when cosecreted with catecholamines during splanchnic stimulation.
Indexed as
Identifiers
8300415PMC1259906W1568712429What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.