ArticleCurrent genetics1993
Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger.
Article in Current genetics, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 77 citations in OpenAlex.
- Rational Design of Mechanism-Based Inhibitors and Activity-Based Probes for the Identification of Retaining α-l-Arabinofuranosidases.Journal of the American Chemical Society · 2020Article
- Insight to Improve α-L-Arabinofuranosidase Productivity inFrontiers in microbiology · 2018Article
- Expression-based clustering of CAZyme-encoding genes of Aspergillus niger.BMC genomics · 2017Article
- Secreted Alpha-N-Arabinofuranosidase B Protein Is Required for the Full Virulence of Magnaporthe oryzae and Triggers Host Defences.PloS one · 2016Article
- Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization.Scientific reports · 2015Article
- Identification and Characterization of a Novel Galactofuranose-Specific β-D-Galactofuranosidase from Streptomyces Species.PloS one · 2015Article
- Overexpression, purification and characterisation of homologous α-L-arabinofuranosidase and endo-1,4-β-D-glucanase in Aspergillus vadensis.Journal of industrial microbiology & biotechnology · 2014Article
- Flavour-active wine yeasts.Applied microbiology and biotechnology · 2012Review
- Mapping the polysaccharide degradation potential of Aspergillus niger.BMC genomics · 2012Article
- d-Xylose concentration-dependent hydrolase expression profiles and the function of CreA and XlnR in Aspergillus niger.Applied and environmental microbiology · 2012Article
- Fungal enzyme sets for plant polysaccharide degradation.Applied microbiology and biotechnology · 2011Review
- Novel bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum.Applied and environmental microbiology · 2010Article
- Aspergillus enzymes involved in degradation of plant cell wall polysaccharides.Microbiology and molecular biology reviews : MMBR · 2001Review
- Purification and substrate specificities of two alpha-L-arabinofuranosidases from Aspergillus awamori IFO 4033.Applied and environmental microbiology · 1998Article
- The transcriptional activator XlnR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger.Applied and environmental microbiology · 1998Article
- Cloning of genes encoding alpha-L-arabinofuranosidase and beta-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae.Applied and environmental microbiology · 1996Article
- A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1,4)-xylosidase activities.Applied and environmental microbiology · 1995Article
- Extracellular arabinases in Aspergillus nidulans: the effect of different cre mutations on enzyme levels.Archives of microbiology · 1994Article
- Molecular cloning, expression and structure of the endo-1,5-alpha-L-arabinase gene of Aspergillus niger.Applied microbiology and biotechnology · 1993Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on amino-acid sequence data from Aspergillus niger alpha-L-arabinofuranosidase B (ABF B), and cyanogen bromide fragments derived thereof, deoxyoligonucleotide mixtures were designed to be employed as primers in a polymerase chain reaction (PCR) on A. niger genomic DNA. This resulted in amplification of three related PCR products. The abfB gene encoding ABF B was isolated from a genomic library using such an amplification product as a probe. A 5.1-kb BamHI fragment was subcloned to result in plasmid pIM991. Upon introduction by co-transformation into both A. niger and A. nidulans uridine auxotrophic strains, pIM991 was shown to contain the functional gene since prototrophic transformants overproduced ABF B upon growth on the inducing carbon source sugar beet pulp. A plate assay was developed enabling quick selection of ABF B-overproducing transformants. The sequence of a 4122-bp long BamHI/SstI fragment was determined. The abfB gene does not contain introns and codes for a protein of 499 amino acids. The mature ABF B, 481 amino acids in length, has a deduced molecular weight of 50.7 kDa. A. niger abfB is the first eukaryotic gene encoding an ABF to be characterized.
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