ArticleThe Journal of cell biology1994
Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 189 citations in OpenAlex.
- Nuclear speckles: a fundamental layer of gene regulation.Trends in cell biology · 2026Review
- Relationships between clusters of interchromatin granules and chromatin fibers.Histochemistry and cell biology · 2026Article
- Phosphorylated-tau associates with HSV-1 chromatin and correlates with nuclear speckles decondensation in low-density host chromatin regions.Neurobiology of disease · 2025Article
- Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.Nature communications · 2025Article
- CLK2 Condensates Reorganize Nuclear Speckles and Induce Intron Retention.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Nuclear speckleopathies: developmental disorders caused by variants in genes encoding nuclear speckle proteins.Human genetics · 2024Review
- CDK11 requires a critical activator SAP30BP to regulate pre-mRNA splicing.The EMBO journal · 2023Article
- Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells.American journal of cancer research · 2023Article
- Mathematical model of structural changes in nuclear speckle.Biophysics and physicobiology · 2023Article
- Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study.Nucleus (Austin, Tex.) · 2022Article
- Recruitment of a splicing factor to the nuclear lamina for its inactivation.Communications biology · 2022Article
- Nuclear speckles - a driving force in gene expression.Journal of cell science · 2022Review
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- Reorganization of the nuclear compartments involved in transcription and RNA processing in myonuclei of type I spinal muscular atrophy.Histochemistry and cell biology · 2019Article
- Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition.Journal of cell science · 2019Article
- The nuclear GSK-3β regulated post-transcriptional processing of mRNA through phosphorylation of SC35.Molecular and cellular biochemistry · 2019Article
- C3G dynamically associates with nuclear speckles and regulates mRNA splicing.Molecular biology of the cell · 2018Article
- Exploring the Crosstalk BetweenFrontiers in genetics · 2018Review
- On the origin of non-membrane-bound organelles, and their physiological function.Journal of theoretical biology · 2017Article
40 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the functional significance of the organization of pre-mRNA splicing factors in a speckled distribution in the mammalian cell nucleus. Upon microinjection into living cells of oligonucleotides or antibodies that inhibit pre-mRNA splicing in vitro, we observed major changes in the organization of splicing factors in vivo. Interchromatin granule clusters became uniform in shape, decreased in number, and increased in both size and content of splicing factors, as measured by immunofluorescence. These changes were transient and the organization of splicing factors returned to their normal distribution by 24 h following microinjection. Microinjection of these oligonucleotides or antibodies also resulted in a reduction of transcription in vivo, but the oligonucleotides did not inhibit transcription in vitro. Control oligonucleotides did not disrupt splicing or transcription in vivo. We propose that the reorganization of splicing factors we observed is the result of the inhibition of splicing in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.