ArticleProceedings of the National Academy of Sciences of the United States of America1994
Mouse limb muscle is determined in the absence of the earliest myogenic factor myf-5.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 127 citations in OpenAlex.
- Regulatory Landscapes of Muscle Satellite Cells: From Mechanism to Application.International journal of stem cells · 2025Review
- Mechanics of Lung Development.Advances in anatomy, embryology, and cell biology · 2023Article
- Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm.PLoS biology · 2019Article
- Fine-tuning the onset of myogenesis by homeobox proteins that interact with the Myf5 limb enhancer.Biology open · 2015Article
- Pax3 synergizes with Gli2 and Zic1 in transactivating the Myf5 epaxial somite enhancer.Developmental biology · 2013Article
- The key enzyme of the sialic acid metabolism is involved in embryoid body formation and expression of marker genes of germ layer formation.International journal of molecular sciences · 2013Article
- Noggin recruits mesoderm progenitors from the dorsal aorta to a skeletal myogenic fate.Developmental biology · 2012Article
- Skeletal myogenesis and Myf5 activation.Transcription · 2011Article
- Origin of vertebrate limb muscle: the role of progenitor and myoblast populations.Current topics in developmental biology · 2011Review
- Sulf1A and HGF regulate satellite-cell growth.Journal of cell science · 2010Article
- PITX2 gain-of-function induced defects in mouse forelimb development.BMC developmental biology · 2008Article
- Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb.Genes & development · 2006Article
- Pax3/Pax7 mark a novel population of primitive myogenic cells during development.Genes & development · 2005Article
- The transcriptional activator PAX3-FKHR rescues the defects of Pax3 mutant mice but induces a myogenic gain-of-function phenotype with ligand-independent activation of Met signaling in vivo.Genes & development · 2003Article
- Defective T-cell activation is associated with augmented transforming growth factor Beta sensitivity in mice with mutations in the Sno gene.Molecular and cellular biology · 2003Article
- The formation of skeletal muscle: from somite to limb.Journal of anatomy · 2003Review
- Article
- Targeted transgenesis.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Myogenin and MEF2 function synergistically to activate the MRF4 promoter during myogenesis.Molecular and cellular biology · 1995Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
myf-5 is the only member of the MyoD family of myogenic regulatory genes to be expressed in the mouse embryo prior to muscle cell differentiation. We have used the developing limb as a model in which to follow the formation of peripheral muscle, to address the question of whether myogenic precursor cells are already present in the limb bud before expression of myf-5. The lacZ reporter gene has been introduced into the myf-5 gene by homologous recombination so that its expression is under the control of the endogenous myf-5 locus. beta-Galactosidase (beta-gal) coloration provides a sensitive assay for myf-5+ cells. Embryos were generated from embryonic stem cells carrying this mutation, and the appearance of beta-gal+ (myf-5+) cells was followed during limb development in vivo. Limb buds, at a stage when they are beta-gal-, were cultured in vitro. After several days, beta-gal+ cells accumulated in the premuscle mass. We conclude that determined muscle precursor cells in the limb bud do not initially express any member of the MyoD family. Furthermore, myogenic precursor cells in the somite, which, according to the avian model, migrate from the ventral/lateral edge of the dermomyotome to form peripheral muscle masses, are also negative for these factors.
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